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Inne dokumenty

11557

Sigma-Aldrich

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

chromogenic, ≥98% (HPLC), powder or crystals

Synonim(y):

AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

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About This Item

Wzór empiryczny (zapis Hilla):
C18H24N6O6S4
Numer CAS:
30931-67-0
Masa cząsteczkowa:
548.68
Beilstein:
7329461
Numer WE:
250-396-6
Numer MDL:
MFCD00010404
Kod UNSPSC:
12352204
Identyfikator substancji w PubChem:
329749485
NACRES:
NA.83

product name

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)

Próba

≥98% (HPLC)

Postać

powder or crystals

zanieczyszczenia

≤6.0% water

rozpuszczalność

H2O: 10 mg/mL, slightly hazy to clear

temp. przechowywania

2-8°C

ciąg SMILES

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

Klucz InChI

OHDRQQURAXLVGJ-AXMZSLBLSA-N

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Zastosowanie

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used:
  • in the radical scavenging assay to test the antioxidant property of Pinus densiflora bark (PDB) extracts
  • to test the antioxidant capacity (AOC) of Aloe vera samples
  • as a substrate for streptavidin-horse radish peroxidase (HRPO) in enzyme-linked immunosorbent assay (ELISA)

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Inne uwagi

Chromogenic substrate for peroxidase; The ABTS-H2O2-peroxidase system in EIA
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Gloves

Certyfikaty analizy (CoA)

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A Szutowicz et al.
Analytical biochemistry, 138(1), 86-94 (1984-04-01)
Monoamine oxidase is assayed in tissue by a colorimetric reaction using horse radish peroxidase and 2,2'-azinodi(ethylbenzthiazoline-6-sulfonic acid to measure H2O2 formed during oxidation of amines. The method has a coefficient of variation of approximately 2.5% and provides results comparable with
Prathik Roy et al.
Journal of hazardous materials, 243, 286-291 (2012-11-21)
We have developed a simple, colorimetric iron telluride (FeTe) nanorods (NRs) based system for the detection of mercury, mainly based on the cation exchange reaction between FeTe NRs and Hg(2+). FeTe NRs (length, 105 ± 21 nm) react with Hg(2+)
A Catarina Guedes et al.
Food chemistry, 138(1), 638-643 (2012-12-26)
A renewed interest in antioxidants has arisen in recent years; microalgae and cyanobacteria are potential sources thereof for use as food/feed ingredients. However, improved methods for comprehensive screening of antioxidant capacity specifically in intracellular extracts of marine microorganisms are required
Yi-Kai Chih et al.
Bioelectrochemistry (Amsterdam, Netherlands), 91, 44-51 (2013-02-19)
An 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)-immobilized carbon nanotube (CNT) electrode was used to simultaneously detect dopamine (DA) and uric acid (UA) in the presence of ascorbic acid (AA) with differential pulse voltammetry. When ABTS was immobilized onto the CNT electrode in the
Jianwei Dong et al.
Journal of bioscience and bioengineering, 118(4), 396-399 (2014-04-20)
The tubers of Bletilla formosana were fermented with eight plant pathogen fungi, respectively, and antioxidant activities and total phenolic content (TPC) of the crude extracts of fermented products and non-fermented products were investigated. The antioxidant activities were evaluated in three
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