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Dokumenty

08445

7-Amino-4-methyl-3-coumarinylacetic acid

Name: 7-Amino-4-methyl-3-coumarinylacetic acid
Brand: SIGMA

BioReagent, suitable for fluorescence, ~90% (HPLC)

Synonim(y):

2H-1-Benzopyran-3-acetic acid, AMCA-H, Aminomethyl coumarin acetic acid

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About This Item

Wzór empiryczny (zapis Hilla):

C₁₂H₁₁NO₄

Numer CAS:

106562-32-7

Masa cząsteczkowa:

233.22

Beilstein:

7927205

Numer MDL:

MFCD00081051

Kod UNSPSC:

12352106

Identyfikator substancji w PubChem:

329748585

NACRES:

NA.32

linia produktu

BioReagent

Próba

~90% (HPLC)

Postać

powder

rozpuszczalność

DMF: soluble
DMSO: soluble
aqueous base: soluble

fluorescencja

λ_ex 350 nm; λ_em 433 nm in methanol

przydatność

suitable for fluorescence

ciąg SMILES

CC1=C(CC(O)=O)C(=O)Oc2cc(N)ccc12

InChI

1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)

Klucz InChI

QEQDLKUMPUDNPG-UHFFFAOYSA-N

Zastosowanie

AMCA is a useful agent for labeling proteins. Demonstrates a characteristic absorption peak at 345 nm with a fluorescence emission at 440-460 nm (blue region); however, when conjugated with proteins the absorption peak shifts to 355 nm with a fluorescence emission at 440-460 nm. It has a high fluorescence yield with a sufficient Stokes shift (100 nm) and is relatively photostable . Relatively easy to activate and couple to the N-terminal amino group of a peptide resulting in an acid stable amide bond .

Opakowanie

Bottomless glass bottle. Contents are inside inserted fused cone.

Inne uwagi

Useful blue fluorophore for immunofluorescence, λ_abs ~350 nm, λ_em ~440 nm

This page may contain text that has been machine translated.

Piktogramy

GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H315,H319,H335

Zwroty wskazujące środki ostrożności

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Klasyfikacja zagrożeń

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Gloves

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Immunodominance of a low-affinity major histocompatibility complex-binding myelin basic protein epitope (residues 111-129) in HLA-DR4 (B1*0401) subjects is associated with a restricted T cell receptor repertoire.

P A Muraro et al.

The Journal of clinical investigation, 100(2), 339-349 (1997-07-15)

The pathogenesis of multiple sclerosis (MS) is currently ascribed in part to a T cell-mediated process targeting myelin components. The T cell response to one candidate autoantigen, myelin basic protein (MBP), in the context of HLA-DR15Dw2, has been previously studied

Two- and three-color immunofluorescence using aminocoumarin, fluorescein, and phycoerythrin-labelled antibodies and single laser flow cytometry.

D Delia et al.

Cytometry, 12(6), 537-544 (1991-01-01)

Antibodies coupled to 7-aminocoumarin (AMCA) emit a bright blue fluorescence under ultraviolet (UV) excitation and are therefore ideal for three-color immunofluorescence (IF) with fluorescein (FITC) and phycoerythrin (PE) labeled reagents; however, due to the different absorption spectra, the use of

Aminomethyl coumarin acetic acid: a new fluorescent labelling agent for proteins.

H Khalfan et al.

The Histochemical journal, 18(9), 497-499 (1986-09-01)

A new fluorescent protein labelling agent, 7-amino-4-methyl coumarin-3-acetic acid (AMCA), emits in the blue region (440-460 nm) on activation with UV light (350 nm). The active reagent is the N-hydroxysuccinimide ester which reacts with lysine residues under mild conditions to

Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences.

P M Nederlof et al.

Cytometry, 10(1), 20-27 (1989-01-01)

A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. Three chromosome-specific repetitive

A simple immunofluorescence technique for simultaneous visualization of mast cells and nerve fibers reveals selectivity and hair cycle--dependent changes in mast cell--nerve fiber contacts in murine skin.

V A Botchkarev et al.

Archives of dermatological research, 289(5), 292-302 (1997-04-01)

Close contacts between mast cells (MC) and nerve fibers have previously been demonstrated in normal and inflamed skin by light and electron microscopy. A key step for any study in MC-nerve interactions in situ is to simultaneously visualize both communication

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