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11815016001
Roche
Macierz powinowactwa anty-HA
from rat IgG1
Synonim(y):
matryca powinowactwa, anty-ha
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
białko sprzężone
agarose conjugate
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
3F10, monoclonal
Formularz
slurry
opakowanie
pkg of 1 mL (settled resin volume)
producent / nazwa handlowa
Roche
izotyp
IgG1
sekwencja epitopowa
YPYDVPDYA
pojemność
2-8 nmol/mL binding capacity
temp. przechowywania
2-8°C
Powiązane kategorie
Opis ogólny
Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)
Specyficzność
Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Zastosowanie
Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
- Western blotting using the Anti-HA antibody
- Silver staining (or similar protein stain)
- In coimmunoprecipitation.
Use Anti-HA Affinity Matrix for:
- Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts
- Affinity column purification of HA-tagged proteins from crude protein extracts
Cechy i korzyści
- Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
- Use nondenaturing conditions for elution of your HA-tagged protein of choice.
- Purify even rarely expressed HA-tagged proteins.
- Highly specific to YPYDVPDYA.
- Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
- Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.
Jakość
Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.
Postać fizyczna
1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.
Komentarz do analizy
Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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Protokoły
Anti-HA Affinity Matrix Protocol & Troubleshooting
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