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11815016001

Roche

Anti-HA Affinity Matrix

from rat IgG1

Synonim(y):

affinity matrix, anti-ha

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About This Item

Kod UNSPSC:
12352203

pochodzenie biologiczne

rat

Poziom jakości

100

białko sprzężone

agarose conjugate

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

3F10, monoclonal

Postać

slurry

opakowanie

pkg of 1 mL (settled resin volume)

producent / nazwa handlowa

Roche

izotyp

IgG1

sekwencja epitopowa

YPYDVPDYA

pojemność

2-8 nmol/mL binding capacity

temp. przechowywania

2-8°C

Opis ogólny

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)

Specyficzność

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Zastosowanie

Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
  • Western blotting using the Anti-HA antibody
  • Silver staining (or similar protein stain)
  • In coimmunoprecipitation.
Use Anti-HA Affinity Matrix for:
  • Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts
  • Affinity column purification of HA-tagged proteins from crude protein extracts

Cechy i korzyści

  • Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
  • Use nondenaturing conditions for elution of your HA-tagged protein of choice.
  • Purify even rarely expressed HA-tagged proteins.
  • Highly specific to YPYDVPDYA.
  • Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
  • Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.

Jakość

Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.

Postać fizyczna

1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.

Komentarz do analizy

Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Francisco José Taberner et al.
BMC cell biology, 11, 47-47 (2010-07-01)
The control of the subcellular localization of cell cycle regulators has emerged as a crucial mechanism in cell division regulation. The active transport of proteins between the nucleus and the cytoplasm is mediated by the transport receptors of the beta-karyopherin
Geetha Hewawasam et al.
Molecular cell, 40(3), 444-454 (2010-11-13)
Cse4 is a variant of histone H3 that is incorporated into a single nucleosome at each centromere in budding yeast. We have discovered an E3 ubiquitin ligase, called Psh1, which controls the cellular level of Cse4 via ubiquitylation and proteolysis.
Abdirahman Abdi et al.
Cellular and molecular life sciences : CMLS, 67(19), 3355-3369 (2010-06-29)
Over the last decade, several protein kinases inhibitors have reached the market for cancer chemotherapy. The kinomes of pathogens represent potentially attractive targets in infectious diseases. The functions of the majority of protein kinases of Plasmodium falciparum, the parasitic protist
David A Hubert et al.
The EMBO journal, 22(21), 5679-5689 (2003-11-01)
The Arabidopsis protein RPM1 activates disease resistance in response to Pseudomonas syringae proteins targeted to the inside of the host cell via the bacterial type III delivery system. We demonstrate that specific mutations in the ATP-binding domain of a single
Stephen C Kales et al.
PloS one, 9(1), e87116-e87116 (2014-01-28)
The Cbl proteins (Cbl, Cbl-b, and Cbl-c) are a highly conserved family of RING finger ubiquitin ligases (E3s) that function as negative regulators of tyrosine kinases in a wide variety of signal transduction pathways. In this study, we identify a
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Protokoły

Anti-HA Affinity Matrix Protocol & Troubleshooting

Anti-HA Affinity Matrix Protocol & Troubleshooting

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