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Key Documents
12158167001
Roche
Anti-HA-Biotin, High Affinity (3F10)
from rat IgG1
Synonim(y):
antibody
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About This Item
Kod UNSPSC:
12352203
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
białko sprzężone
biotin conjugate
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
3F10, monoclonal
Postać
lyophilized (stabilized)
opakowanie
pkg of 50 μg
producent / nazwa handlowa
Roche
izotyp
IgG1
sekwencja epitopowa
YPYDVPDYA
temp. przechowywania
2-8°C
Powiązane kategorie
Opis ogólny
Anti-HA-Biotin, High Affinity (3F10) is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to biotin. The Anti-HA-Biotin, High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5. It is a monoclonal antibody whose high affinity and low working concentrations result in less cross-reactivity than with other antibodies to the HA-epitope. Anti-HA-Biotin, High Affinity (3F10) is a biotin conjugate of this clone which is specifically useful in western blotting, ELISA applications and assays using the universal biotin-streptavidin platform, by allowing specific and highly sensitive detection of HA-tagged proteins.
Specyficzność
Anti-HA-Biotin, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Zastosowanie
Anti-HA-Biotin, High Affinity (3F10) is used for the detection of HA-tagged recombinant proteins using:
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.
- Dot blots
- ELISA (enzyme-linked immunosorbent assay)
- Western blots
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.
Jakość
Function test: The Anti-HA-Biotin; High Affinity is function tested by Western blot analysis of a HA-tagged fusion protein.
Uwaga dotycząca przygotowania
Sample Materials
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:
- Bacterial extracts: 20 mM Tris, pH 8.0, 100 mM NaCl, cOmplete Protease Inhibitor Cocktail Tablets, followed by freeze-thaw.
- Mammalian extracts: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.1% Nonidet P40, complete Protease Inhibitor Cocktail Tablets.
- Other cell lysis buffers may be more appropriate for individual applications. In general, to obtain optimal performance of the affinity matrix:
- Use protease inhibitors to reduce proteolytic activity. Use complete Protease Inhibitor Cocktail Tablets for most applications.
- Limit detergent to the lowest concentration levels necessary to obtain adequate cell lysis.
Working concentration: Working concentration of conjugate depends on application and substrate
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- Dot blot: 100 ng/ml
- ELISA: 100 ng/ml
- Western blot: 100 ng/ml
Rekonstytucja
Add 1 ml double-distilled water to a final concentration of 50 μg/ml.
Rehydrate for 10 minutes prior to use.
Rehydrate for 10 minutes prior to use.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 3 - Skin Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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