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Kluczowe dokumenty
11814460001
Roche
Anti-GFP
from mouse IgG1κ (clones 7.1 and 13.1)
Synonim(y):
anti-green fluorescent protein
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
13.1, monoclonal
7.1, monoclonal
Próba
>90% (HPLC)
Postać
lyophilized
opakowanie
pkg of 200 μg
producent / nazwa handlowa
Roche
izotyp
IgG1κ
temp. przechowywania
2-8°C
Opis ogólny
Green Fluorescent Protein (GFP) is a spontaneously fluorescent 27kDa protein originally isolated from the jellyfish Aequorea victoria. The molecular cloning of the GFP gene and its subsequent expression in heterologous systems has established GFP as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. Since, GFP requires no additional substrates or cofactors, GFP′s green fluorescence can be easily detected using blue or UV light after expression in either prokaryotic or eukaryotic cells. In addition, several mutant forms of GFP with unique spectral properties (e.g., enhanced fluorescence signal and shifts in excitation and emission spectra) have been reported.
Mixture of two high-affinity monoclonal antibodies selected for their performance in detection of GFP and GFP fusion proteins.
Specyficzność
Subtype: Both clones are Mouse IgG1κ
Zastosowanie
Monoclonal antibody for detection of both wild-type and mutant forms of GFP or GFP fusions using:
- Immunoprecipitation
- Western blots
- Immunostaining
Cechy i korzyści
Contents
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200μg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200μg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).
Jakość
Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.
Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.
Uwaga dotycząca przygotowania
Working concentration: Working concentration of antibody depends on application and substrate.
The following concentrations should be taken as a guideline:
Storage conditions (working solution): -15 to -25 °C
The following concentrations should be taken as a guideline:
- Western blot: 1:1000 dilution
- Immunoprecipitation: 2 to 10 μg
Storage conditions (working solution): -15 to -25 °C
Rekonstytucja
Add 500 μl double distilled water to a final concentration of 0.4 mg/ml.
Rehydrate on ice for 30 minutes.
Rehydrate on ice for 30 minutes.
Informacje prawne
This product is sold under license from Columbia University. Rights to use this product are limited to research use only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Columbia Innovation Enterprise, Columbia University, Engineering Terrace - Suite 363, new York, New York 10027.
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Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Certyfikaty analizy (CoA)
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