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Merck

SAB2702214

Sigma-Aldrich

Monoclonal Anti-RFP antibody produced in mouse

clone GT1433, affinity isolated antibody

Synonim(y):

Mouse Anti-Ref Fluorescent protein

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

GT1433, monoclonal

Postać

buffered aqueous solution

masa cząsteczkowa

27kDa

stężenie

0.98mg/mL

metody

immunoprecipitation (IP): suitable
western blot: 5000-20000

izotyp

IgG1

Warunki transportu

wet ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

Red fluorescent protein (RFP) is obtained from the Discosoma coral. It can be used as a fluorescent probe for optical microscopy and acts as a biological marker for biomolecule imaging. RFPs exhibit fluorescence emission beyond 57 0nm. They have lower light-scattering, low photo-toxicity and less autofluorescence interference of cells. RFPs are extremely useful for whole body and deep tissue imaging.

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP.

Zastosowanie

Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Monoclonal Anti-RFP antibody produced in mouse has been used in chromatin immunoprecipitation (ChIP) assay.

Cechy i korzyści

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Inne uwagi

Purification: Affinity purified by Protein G

Postać fizyczna

Phosphate-buffered saline, no preservative added.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast*
Pradeep Kumar Yadav and Ram Rajasekharan
The Journal of Biological Chemistry (2016)
DNA mimics of red fluorescent proteins (RFP) based on G-quadruplex-confined synthetic RFP chromophores
Nucleic Acids Research, 45(18) (2017)
A monomeric red fluorescent protein
Robert E. Campbell
Proceedings of the National Academy of Sciences of the USA (2002)
Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry
Kiryl D
Methods in Cell Biology (2011)
Pradeep Kumar Yadav et al.
The Journal of biological chemistry, 291(35), 18562-18581 (2016-07-13)
The DDHD domain-containing proteins, which belong to the intracellular phospholipase A1 (iPLA1) family, have been predicted to be involved in phospholipid metabolism, lipid trafficking, membrane turnover, and signaling. Defective cardiolipin (CL), phosphatidylethanolamine, and phosphatidylglycerol remodeling cause Barth syndrome and mitochondrial

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