P6611

HIS-Select^® Nickel Affinity Gel

(1:1 suspension in a 20% ethanol solution)

• Synonim(y): Ni-NTA resin, nickel charged agarose
• Kod UNSPSC: 12352200
• NACRES: NA.56

Właściwości

• białko sprzężone: magnetic beads
• Poziom jakości: 200
• Postać: (1:1 suspension in a 20% ethanol solution)
• Właściwości: hydrophilic
• opakowanie: pkg of 1 mL; pkg of 100 mL; pkg of 25 mL; pkg of 5 mL; pkg of 500 mL
• stężenie: 1.5-2.4 mL/mL (suspension in packed gel)
• metody: protein purification: suitable
• kolor: faint blue to very dark blue
• macierz: 6% Beaded Agarose
• pojemność: >15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
• temp. przejścia: flash point 32 °C (closed cup)
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

HIS-Select^® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.

HIS-Select^® Nickel Affinity Gel is durable and can capture the recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with histidine tags are bound using either native or denaturing conditions.

Zastosowanie

HIS-Select^® Nickel Affinity Gel has been used in the purification of recombinant proteins like EF-hand calcium-binding protein (S100A14), LIM homeobox transcription factor 1 alpha protein, sigma-1 receptor as well as harpin, stable protein 1 (SP1), and BCR-ABL fusion protein.

Cechy i korzyści

• High selectivity for higher purity.
• Unique non-charged hydrophilic linkage reduces non-specific binding.
• Binding capacity for histidine-tagged protein is greater than 15 mg/mL.
• Binding under denaturing or non-denaturing conditions.
• One-step purification.
• Minimizes unwanted ionic interactions.
• Minimal nickel leaching.
• Bead size: 45-165 μm.

Powiązanie

It is also available with the EZview™ technology (Product Code E3528).

Postać fizyczna

1:1 suspension in a 20% ethanol solution

Przechowywanie i stabilność

HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer.

Informacje prawne

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H226
• Zwroty wskazujące środki ostrożności: P210 - P233 - P240 - P241 - P242 - P243
• Klasyfikacja zagrożeń: Flam. Liq. 3
• Kod klasy składowania: 3 - Flammable liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): 89.6 °F - closed cup
• Temperatura zapłonu (°C): 32 °C - closed cup