Przejdź do zawartości
Merck
Wszystkie zdjęcia(2)

Key Documents

View All Documentation

11585762001

Roche

DIG Wash and Block Buffer Set

greener alternative

storage temp.:2-8°C

Synonim(y):

wash and block buffer set, dig

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(2)

About This Item

Kod UNSPSC:
41105326

Poziom jakości

100

producent / nazwa handlowa

Roche

charakterystyka ekologicznej alternatywy

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

kategoria ekologicznej alternatywy

, Aligned

temp. przechowywania

2-8°C

Powiązane kategorie

Opis ogólny

Except for the Blocking solution, the 10x concentrated buffers are diluted to the desired concentrations with sterile double-distilled water (not included).
Washing, blocking and detection buffers (10x concentrated) for the immunological detection of DIG - (digoxigenin-)labeled probes.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Zastosowanie

The DIG Wash and Block Buffer Set is used at various steps of DIG hybridization and DIG detection.

Opakowanie

1 set containing 4 components.

Jakość

The buffers are autoclaved, filtered and tested for the absence of DNases and RNases according to the current Quality Control procedures. The buffers are function tested according to the standard procedure of the DIG Nucleic Acid Detection Kit.

Uwaga dotycząca przygotowania

Working solution: dATP, dGTP, dTTP mixture:
For one labeling reaction pipette:
1 μl dATP, (vial 2)
1 μl dGTP, (vial 4)
1 μl dTTP, (vial 5)
to a reaction vial.

Note: If the same type of labeled deoxyribonucleoside-triphosphate is used repeatedly, we recommend the preparation of a mixture of equal parts of the other three deoxyribonucleoside-triphosphates for convenience.
Storage conditions (working solution):
  • After the first usage, the buffers should be stored further at 2 to 8 °C. We recommend to store the concentrated Blocking solution in aliquots at -15 to -25 °C.
  • When Autoclaved: Stock solution can be stored for several days to a week either unopened at RT or at 2 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.

Tylko elementy zestawu

Numer produktu
Opis
  • Washing Buffer 10x concentrated
  • Maleic acid Buffer 10x concentrated
  • Blocking Solution 10x concentrated
  • Detection Buffer 10x concentrated

Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H315,H319

Klasyfikacja zagrożeń

Eye Irrit. 2 - Skin Irrit. 2

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicabledoes not flash

Temperatura zapłonu (°C)

Not applicabledoes not flash

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Shiho Torii et al.
Cell reports, 35(3), 109014-109014 (2021-04-12)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 (COVID-19). Although multiple mutations have been observed in SARS-CoV-2, functional analysis of each mutation of SARS-CoV-2 has been limited by the lack
James A Baum et al.
Journal of economic entomology, 105(2), 616-624 (2012-05-23)
The plant bugs Lygus hesperus Knight (Hemiptera: Miridae) and L. lineolaris (Palisot de Beauvois) have emerged as economic pests of cotton in the United States. These hemipteran species are refractory to the insect control traits found in genetically modified commercial
Dan Sun et al.
Oncology letters, 20(4), 8-8 (2020-08-11)
Since human bladder cancer (BC) is a common malignancy of the urinary system with poor prognosis, it is crucial to clarify the molecular mechanisms of BC development and progression. To the best of our knowledge, the current study demonstrated for
Siqian Feng et al.
Genetics, 217(3) (2021-03-28)
We describe a simple and efficient technique that allows scarless engineering of Drosophila genomic sequences near any landing site containing an inverted attP cassette, such as a MiMIC insertion. This two-step method combines phiC31 integrase-mediated site-specific integration and homing nuclease-mediated
Stefanie Wehner et al.
RNA biology, 11(11), 1467-1478 (2014-12-09)
6S RNA is a highly abundant small non-coding RNA widely spread among diverse bacterial groups. By competing with DNA promoters for binding to RNA polymerase (RNAP), the RNA regulates transcription on a global scale. RNAP produces small product RNAs derived
Wyświetl wszystkie powiązane dokumenty

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej