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12041677001

Roche

CDP-Star®, ready-to-use

>98%, solution, suitable for dot blot, suitable for Northern blotting, suitable for Southern blotting

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About This Item

Kod UNSPSC:
12352204

opis

Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate

Poziom jakości

100

Próba

>98%

Postać

solution

opakowanie

bottle of 2 × 50 mL ([0.25 mM])

producent / nazwa handlowa

Roche

metody

Northern blotting: suitable
Southern blotting: suitable
dot blot: suitable

temp. przechowywania

2-8°C

publikacja

Purity: Purified by HPLC.

Zastosowanie

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
  • Southern blot
  • Northern blot
  • Dot blot
  • Colony and plaque hybridization
  • Gel shift assays
Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
  • Southern blot
  • Northern blot
  • Dot blot
  • Colony and plaque hybridization
  • Gel shift assays
  • Chemiluminescent detection.

Cechy i korzyści

Contents
0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution
CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
  • Save time with a fast, ultra-sensitive substrate.
  • Easily strip and reprobe membranes.
  • Take multiple exposures of up to two days for each experiment.
Reuse the substrate by filtering and storing in sodium azide.

Uwaga dotycząca przygotowania

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

Inne uwagi

  • First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
  • No preincubation step required.
  • Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
  • Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
  • Exposure times are shorter than with CSPD.
  • Do not use nitrocellulose membranes.
Repeat exposures can be made up to two days after the addition of substrate.
For life science research only. Not for use in diagnostic procedures.

Informacje prawne

CDP-Star is a registered trademark of Tropix, Inc.
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

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Odwiedź Bibliotekę dokumentów

Non-Radioactive Detection of Trinucleotide Repeat Size Variability.
Tome S, et al.
PLoS Currents, 6 (2014)
Detection of expanded RNA repeats using thermostable group II intron reverse transcriptase.
Carrell ST, et al.
Nucleic Acids Research, 46(1), e1-e1 (2017)
Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum).
Lin CY, et al.
Plant methods, 13(1), 113-113 (2017)
Ivano Legnini et al.
Nature methods, 16(9), 879-886 (2019-08-07)
Although messenger RNAs are key molecules for understanding life, until now, no method has existed to determine the full-length sequence of endogenous mRNAs including their poly(A) tails. Moreover, although non-A nucleotides can be incorporated in poly(A) tails, there also exists
Omer Choresh et al.
Biomacromolecules, 10(10), 2852-2856 (2009-09-08)
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