11373242910

Fluorescein-12-dUTP

≥85% (HPLC), suitable for ELISA, suitable for hybridization, solution

• Synonim(y): Fluorescein-12-dUTP, dUTP
• Kod UNSPSC: 41116100

Właściwości

• Poziom jakości: 100
• Próba: ≥85% (HPLC)
• Postać: solution
• masa cząsteczkowa: M_r 1018.4 (fluorescein-12-dUTP-Li₄)
• opakowanie: pkg of 25 μL (25 nmol; 1mM)
• producent / nazwa handlowa: Roche
• metody: ELISA: suitable; hybridization: suitable
• temp. przechowywania: −20°C

Opis

Zastosowanie

Fluorescein-12-dUTP is used as a substrate for:

• terminal transferase
• DNA polymerase I (holoenzyme and Klenow fragment),
• Taq DNA polymerase
• reverse transcriptase (e.g., from AMV and M-MuLV).

Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

Jakość

Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%).

Postać fizyczna

Fluorescein-12-dUTP, tetralithium salt.

Przechowywanie i stabilność

The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash