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DIUTP-RO
Roche
Digoxigenin-11-dUTP, alkali-labile
=85% (HPLC), solution, pkg of 25 μL (11573152910 [1 mM]), pkg of 125 μL (11573179910 [1 mM]]])
Synonim(y):
Digoxigenin-11-dUTP, alkali-labile, digoxigenin
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About This Item
Kod UNSPSC:
41105500
Polecane produkty
Poziom jakości
Próba
85% (HPLC)
Postać
solution
opakowanie
pkg of 125 μL (11573179910 [1 mM]]])
pkg of 25 μL (11573152910 [1 mM])
producent / nazwa handlowa
Roche
temp. przechowywania
−20°C
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Opis ogólny
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP), alkali-labile is provided as in 1 mM tetralithium salt, solution. In which DIG is bound to dUTP via an alkali-sensitive ester linkage.
Zastosowanie
Digoxigenin-11-dUTP, alkali-labile can be used for non-radioactive DNA labeling e.g. random primed or nick translation. It can also be used for labeling probes which are preferred in hybridization experiments where stripping and reprobing of the membrane is intended.
Działania biochem./fizjol.
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphophate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.
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Numer produktu
Opis
Cennik
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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