MABT545

Anti-Lamin A and C Antibody, clone 2F4.1

clone 2F4.1, from mouse

• Synonim(y): LMNA
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 2F4.1, monoclonal
• reaktywność gatunkowa: human, rat
• opakowanie: antibody small pack of 25 μg
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_001002016
• numer dostępu UniProt: P48679
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

Lamin A/C (UniProt: P48679; also known as LMNA) is encoded by the Lmna (also known as Lmn1) gene in rat. Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A. Cleavage of 15 residues (aa 647-662) by ZMPSTE24/FACE1 generates the final protein product. Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery. Prelamin-A/C can accelerate smooth muscle cell senescence. It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence. Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system. Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body. (Ref.: Casasola, A., et al. (2016). Nucleus 7(1); 84-102).

Specyficzność

Clone 2F4.1 detects Lamin A/C in human and rat tissues. It targets an epitope within 148 amino acids from the internal region.

Immunogen

GST/His-tagged recombinant fragment corresponding to 148 amino acids from the internal region of rat Lamin A/C.

Zastosowanie

Anti-Lamin A and C, clone 2F4.1, Cat. No. MABT545,is a mouse monoclonal antibody that detects Lamin A/C and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Lamin A and C in rat cerebral cortex, rat hippocampus, rat cerebellum, and human liver tissue sections.

Research Category
Cell Structure

Jakość

Evaluated by Western Blotting in WI-38 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Lamin A and C in WI-38 cell lysate.

Opis wartości docelowych

~74/65 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1