MABN844
Anti-Neurophysin 1 Antibody, clone PS 38
clone PS 38, from mouse
Synonim(y):
Oxytocin-neurophysin 1, OT-NPI, Oxytocin, Ocytocin, Neurophysin 1
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
PS 38, monoclonal
reaktywność gatunkowa
rat
metody
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable
izotyp
IgG2bκ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
rat ... Oxt(25504)
Opis ogólny
Neurophysins are linear cycteine-rich proteins containing 93-95 amino acids. Two types of neurophysins, NPI and NPII are found in most mammalian species and they serve as carriers of oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormone is synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or VAP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI), or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. VAP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood. VAP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).
Specyficzność
Clone PS 38 detects neurophysin 1 (oxytocin-associated NP), but not ocytocin/oxytocin or neurophysin 2 (vasopressin-associated NP)
Immunogen
Epitope: Neurophysin 1
KLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes corresponding to rat Neurophysin 1.
Zastosowanie
Immunohistochemistry: A representative lot detected oxytocin (OT)-synthesizing neurons in the hypothalamic supraoptic (SON) and neural lobe (NL) regions of paraffin-embedded rat hypothalamus and pituitary tissue sections (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Radioimmunoassay (RIA): A representative lot was assessed for binding affinity toward purified rat neurophysinn 1 by RIA (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Western Blotting Analysis: A representative lot detected neurophysinn 1 in rat posterior pituitary extracts (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Radioimmunoassay (RIA): A representative lot was assessed for binding affinity toward purified rat neurophysinn 1 by RIA (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Western Blotting Analysis: A representative lot detected neurophysinn 1 in rat posterior pituitary extracts (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Neurophysin 1 Antibody, clone PS 38 is validated for use in Western Blotting, Immunohistochemistry, Radioimmunoassay for the detection of Neurophysin 1.
Jakość
Evaluated by Western Blotting in rat pituitary tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 1 in 10 µg of rat pituitary tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 1 in 10 µg of rat pituitary tissue lysate.
Opis wartości docelowych
~12 kDa observed
Postać fizyczna
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Dipanwita Pati et al.
Journal of neuroendocrinology, 32(3), e12839-e12839 (2020-03-07)
Significant prior evidence indicates that centrally acting oxytocin robustly modulates stress responsiveness and anxiety-like behaviour, although the neural mechanisms behind these effects are not entirely understood. A plausible neural basis for oxytocin-mediated stress reduction is via inhibition of corticotrophin-releasing hormone
Fei Zeng et al.
ASN neuro, 13, 17590914211055064-17590914211055064 (2021-11-24)
This study investigated the effects of the pharmacological manipulation of noradrenergic activities on dopaminergic phenotypes in aged rats. Results showed that the administration of L-threo-3,4-dihydroxyphenylserine (L-DOPS) for 21 days significantly increased the expression of tyrosine hydroxylase (TH) and dopamine transporter
Marie Habart et al.
STAR protocols, 4(1), 101968-101968 (2023-01-05)
Here, we present an optimized iDISCO+ protocol combining tissue clearing and light sheet microscopy to map the postnatal development of oxytocin and vasopressin neurons in mouse hypothalamus. We describe tissue preparation, immunostaining, clearing, and imaging. We then detail how to
Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies.
Ben-Barak, Y; Russell, JT; Whitnall, MH; Ozato, K; Gainer, H
The Journal of Neuroscience null
Dongyang Li et al.
ASN neuro, 12, 1759091420944658-1759091420944658 (2020-09-24)
Oxytocin, a hypothalamic neuropeptide essential for breastfeeding, is mainly produced in oxytocin neurons in the supraoptic nucleus (SON) and paraventricular nucleus. However, mechanisms underlying oxytocin secretion, specifically the involvement of hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) in oxytocin neuronal activity
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