MABE132

Anti-MyoD Antibody, clone 5F11

clone 5F11, from rat

• Synonim(y): Myoblast determination protein 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rat
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 5F11, monoclonal
• reaktywność gatunkowa: mouse
• metody: immunocytochemistry: suitable; western blot: suitable
• izotyp: IgG2aκ
• numer dostępu NCBI: NP_034996
• numer dostępu UniProt: P10085
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: mouse ... Myod1(17927)

Opis

Opis ogólny

Myoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5.

Immunogen

GST-tagged recombinant protein corresponding to mouse MyoD.

Zastosowanie

Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells.

Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Jakość

Evaluated by Western Blot in C2C12 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.

Opis wartości docelowych

~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated.

Powiązanie

Replaces: MABE514

Postać fizyczna

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
C2C12 cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable