MAB5366
Anti-Reelin Antibody, a.a. 164-189 mreelin, clone 142
clone 142, Chemicon®, from mouse
Synonim(y):
reelin
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
142, monoclonal
reaktywność gatunkowa
mouse, vertebrates, human
reaktywność gatunkowa (przewidywana na podstawie homologii)
rat
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry: suitable
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... RELN(5649)
Opis ogólny
The highly layered structure of the cerebral cortex is established through the pattern of neuronal cell migrations. The first step is the creation of the primordial layer, the preplate, consisting of radial glial cells and the earliest generated neurons. Among these neurons are the Cajal-Retzius neurons. In the next step, the preplate splits into a superficial (marginal) zone, where the Cajal-Retzius neurons reside, and a deep subplate wherein the neurons form. Neurons migrating from the subplate form the cortical plate. This migration takes place on the radial glial fibers.
The reeler mutant in mouse displays an abnormal pattern of cell migration throughout the cerebral and cerebellar cortices. The preplate forms normally, and the neurons differentiate at the correct times in the ventricular zone. However, instead of forming the normal "inside-out" arrangement of neurons in the cortical plate, the older neurons are found furthest from the ventricular zone, while the younger neurons do not migrate far at all. The reeler cerebral cortex is inverted from that of the wild type mouse.
The defect of the reeler mice appears to be in the production of an extracellular matrix protein by the Cajal-Retzius cells (D′Arcangelo et al., 1995, Nature 374:719-723.; Ogawa et al., 1995 Neuron 14:899-912.) This 388kDa protein is made by wild-type mice but not by the reeler mutants. It is thought that this Reelin protein is crucial for positioning the migrating neuron within the cortical plate (Figure 1). In the absence of Reelin, the migrating neuron would be "lost," and the cortical plate would be abnormal. We do not yet know the mechanisms by which Reelin informs the cells as to their position, how the cell responds to Reelin, and why the absence of reelin should give an "inverted" plate. However, the identification of the protein encoded by the reeler gene should allow us to begin these studies.
The reeler mutant in mouse displays an abnormal pattern of cell migration throughout the cerebral and cerebellar cortices. The preplate forms normally, and the neurons differentiate at the correct times in the ventricular zone. However, instead of forming the normal "inside-out" arrangement of neurons in the cortical plate, the older neurons are found furthest from the ventricular zone, while the younger neurons do not migrate far at all. The reeler cerebral cortex is inverted from that of the wild type mouse.
The defect of the reeler mice appears to be in the production of an extracellular matrix protein by the Cajal-Retzius cells (D′Arcangelo et al., 1995, Nature 374:719-723.; Ogawa et al., 1995 Neuron 14:899-912.) This 388kDa protein is made by wild-type mice but not by the reeler mutants. It is thought that this Reelin protein is crucial for positioning the migrating neuron within the cortical plate (Figure 1). In the absence of Reelin, the migrating neuron would be "lost," and the cortical plate would be abnormal. We do not yet know the mechanisms by which Reelin informs the cells as to their position, how the cell responds to Reelin, and why the absence of reelin should give an "inverted" plate. However, the identification of the protein encoded by the reeler gene should allow us to begin these studies.
Specyficzność
Reelin. The epitope is between amino acids 164 and 189.
Immunogen
Epitope: a.a. 164-189
Recombinant reelin amino acids 40-189.
Zastosowanie
Research Category
Neuroscience
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Growth Cones & Axon Guidance
This Anti-Reelin Antibody, a.a. 164-189 mreelin, clone 142 is validated for use in IH, WB for the detection of Reelin.
Western blot:
1:250-1:500, In Western blots, reelin appears as three bands ~400 - 450, 300, and 180 - 200 kDa′s.
Immunohistochemistry:
1:250-1:500, 4% PFA fixed tissues. A previous lot of this antibody was used in IH.
Optimal working dilutions must be determined by the end user.
1:250-1:500, In Western blots, reelin appears as three bands ~400 - 450, 300, and 180 - 200 kDa′s.
Immunohistochemistry:
1:250-1:500, 4% PFA fixed tissues. A previous lot of this antibody was used in IH.
Optimal working dilutions must be determined by the end user.
Jakość
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Reelin on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Reelin on 10 μg of mouse brain lysates.
Opis wartości docelowych
In Western blots, reelin can appear as three bands ~400 - 450, 300, and 180 - 200 kDa′s.
Postać fizyczna
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
Przechowywanie i stabilność
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Komentarz do analizy
Control
Mouse liver or kidney
Mouse liver or kidney
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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