MAB5502

Anti-Vesicular Glutamate Transporter 1 Antibody

CHEMICON^®, mouse monoclonal, 3C10.2

• Synonim(y): Solute carrier family 17 member 7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, vGluT1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• Nazwa produktu: Anti-Vesicular Glutamate Transporter 1 Antibody, clone 3C10.2, Chemicon^®, from mouse
• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 3C10.2, monoclonal
• reaktywność gatunkowa: mouse
• reaktywność gatunkowa (przewidywana na podstawie homologii): rat
• producent / nazwa handlowa: Chemicon^®
• metody: immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG1
• numer dostępu NCBI: NP_446311
• numer dostępu UniProt: Q62634
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: rat ... Slc17A7(116638)

Opis

Opis ogólny

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate. Vesicular glutamate transporters pack the neurotransmitter into synaptic vesicles so that they can be released into the synapse. VGLUTs are dependent on a proton gradient that they create by hydrolysing adenosine triphosphate (ATP). VGLUTs have only between one hundredth and one thousandth the affinity for glutamate that EAATs have.

Specyficzność

Reacts with VGLUT1 (Vesicular Glutamate Transporter 1).

Immunogen

Recombinant protein from rat VGLUT1.

Zastosowanie

Immunohistochemistry:
A previous lot of this antibody was tested on IH.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Neuronal & Glial Markers

This Anti-Vesicular Glutamate Transporter 1 Antibody, clone 3C10.2, Cat. No. MAB5502, is validated for use in IH, WB for the detection of VGluT1.

Jakość

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Vesicular Glutamate Transporter 1 on 10 μg of Mouse Brain lysates.

Opis wartości docelowych

60 kDa

Postać fizyczna

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing PBS containing 0.1% sodium azide.

Przechowywanie i stabilność

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

Komentarz do analizy

Control
Brain

VGLUT1 Control Peptide AG208

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable