MAB5364
Anti-Reelin Antibody, a.a. 164-496 mreelin, clone G10
clone G10, Chemicon®, from mouse
Synonim(y):
Anti-ETL7, Anti-LIS2, Anti-PRO1598, Anti-RL
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
G10, monoclonal
reaktywność gatunkowa
rodent, mouse, rat
reaktywność gatunkowa (przewidywana na podstawie homologii)
human
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry: suitable
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... RELN(5649)
Opis ogólny
The highly layered structure of the cerebral cortex is established through the pattern of neuronal cell migrations. The first step is the creation of the primordial layer, the preplate, consisting of radial glial cells and the earliest generated neurons. Among these neurons are the Cajal-Retzius neurons. In the next step, the preplate splits into a superficial (marginal) zone, where the Cajal-Retzius neurons reside, and a deep subplate wherein the neurons form. Neurons migrating from the subplate form the cortical plate. This migration takes place on the radial glial fibers.
The reeler mutant in mouse displays an abnormal pattern of cell migration throughout the cerebral and cerebellar cortices. The preplate forms normally, and the neurons differentiate at the correct times in the ventricular zone. However, instead of forming the normal "inside-out" arrangement of neurons in the cortical plate, the older neurons are found furthest from the ventricular zone, while the younger neurons do not migrate far at all. The reeler cerebral cortex is inverted from that of the wild type mouse.
The defect of the reeler mice appears to be in the production of an extracellular matrix protein by the Cajal-Retzius cells (D′Arcangelo et al., 1995, Nature 374:719-723.; Ogawa et al., 1995 Neuron 14:899-912.) This 388kDa protein is made by wild-type mice but not by the reeler mutants. It is thought that this Reelin protein is crucial for positioning the migrating neuron within the cortical plate (Figure 1). In the absence of Reelin, the migrating neuron would be "lost," and the cortical plate would be abnormal. We do not yet know the mechanisms by which Reelin informs the cells as to their position, how the cell responds to Reelin, and why the absence of reelin should give an "inverted" plate. However, the identification of the protein encoded by the reeler gene should allow us to begin these studies.
The reeler mutant in mouse displays an abnormal pattern of cell migration throughout the cerebral and cerebellar cortices. The preplate forms normally, and the neurons differentiate at the correct times in the ventricular zone. However, instead of forming the normal "inside-out" arrangement of neurons in the cortical plate, the older neurons are found furthest from the ventricular zone, while the younger neurons do not migrate far at all. The reeler cerebral cortex is inverted from that of the wild type mouse.
The defect of the reeler mice appears to be in the production of an extracellular matrix protein by the Cajal-Retzius cells (D′Arcangelo et al., 1995, Nature 374:719-723.; Ogawa et al., 1995 Neuron 14:899-912.) This 388kDa protein is made by wild-type mice but not by the reeler mutants. It is thought that this Reelin protein is crucial for positioning the migrating neuron within the cortical plate (Figure 1). In the absence of Reelin, the migrating neuron would be "lost," and the cortical plate would be abnormal. We do not yet know the mechanisms by which Reelin informs the cells as to their position, how the cell responds to Reelin, and why the absence of reelin should give an "inverted" plate. However, the identification of the protein encoded by the reeler gene should allow us to begin these studies.
Specyficzność
Reelin
The antibody shows weak reactivity to reelin from other species.
Immunogen
Epitope: a.a. 164-496 mreelin
Recombinant reelin amino acids 164-496
Zastosowanie
Detect Reelin using this Anti-Reelin Antibody, a.a. 164-496 mreelin, clone G10 validated for use in IH & WB.
Immunohistochemistry: A previous lot of this antibody was used in IH.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Growth Cones & Axon Guidance
Jakość
Evaluated by Western Blot on Rat brain lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected reelin on 10 μg of Rat brain lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected reelin on 10 μg of Rat brain lysates.
Opis wartości docelowych
~388 kDa
Postać fizyczna
Format: Purified
Mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer with 0.25 M NaCl and 0.1% sodium azide.
Protein A purified
Przechowywanie i stabilność
Stable for 1 year at 2-8ºC from date of receipt.
Komentarz do analizy
Control
Mouse liver, kidney, rat brain lysate
Mouse liver, kidney, rat brain lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Breaches of the pial basement membrane and disappearance of the glia limitans during development underlie the cortical lamination defect in the mouse model of muscle-eye-brain disease.
Huaiyu Hu, Yuan Yang, Amber Eade, Yufang Xiong, Yue Qi
The Journal of Comparative Neurology null
Early serotonergic projections to Cajal-Retzius cells: relevance for cortical development.
Janusonis, S; Gluncic, V; Rakic, P
The Journal of Neuroscience null
Origins and migratory routes of murine Cajal-Retzius cells.
Fernando Garcia-Moreno,Laura Lopez-Mascaraque,Juan A De Carlos
The Journal of Comparative Neurology null
Localization of the transcriptional coactivator PGC-1alpha to GABAergic neurons during maturation of the rat brain.
Rita Marie Cowell,Kathryn Rose Blake,James W Russell
The Journal of Comparative Neurology null
Gülşen Sürmeli et al.
Neuron, 88(5), 1040-1053 (2015-11-27)
Deep layers of the medial entorhinal cortex are considered to relay signals from the hippocampus to other brain structures, but pathways for routing of signals to and from the deep layers are not well established. Delineating these pathways is important
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej