MABE253
Anti-Ago2 Antibody, clone 11A9
clone 11A9, from rat
Synonim(y):
Protein argonaute-2, Argonaute2, hAgo2, Eukaryotic translation initiation factor 2C 2, eIF-2C 2, eIF2C 2, PAZ Piwi domain protein, PPD, Protein slicer
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
11A9, monoclonal
reaktywność gatunkowa
human
metody
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG2aκ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... AGO2(27161)
Opis ogólny
Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).
Immunogen
Linear peptide corresponding to human Ago2.
Zastosowanie
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Ago2 in HEK239 cell lysate (Rudel, S., et al. (2008) RNA. 14(6):1244-1253.).
Immunocytochemistry Analysis: A represeentative lot from an independent laboratory detected Ago2 in HEK293, HeLa, and RPE-1 cells (Weinmann, L., et al. (2009). Cell. 136(3):496-507.).
Immunocytochemistry Analysis: A represeentative lot from an independent laboratory detected Ago2 in HEK293, HeLa, and RPE-1 cells (Weinmann, L., et al. (2009). Cell. 136(3):496-507.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Metabolism & Binding Proteins
Use Anti-Ago2 Antibody, clone 11A9 (Rat Monoclonal Antibody) validated in WB, IP, ICC to detect Ago2 also known as Protein argonaute-2, Argonaute2, hAgo2, eIF-2C 2.
Jakość
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected Ago2 in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected Ago2 in 10 µg of HeLa cell lysate.
Opis wartości docelowych
~85 kDa observed. Two isoforms at ~97 kDa and ~94 kDa are known to exist.
Postać fizyczna
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Komentarz do analizy
Control
HeLa cell lysate
HeLa cell lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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