MAB4072
Anti-BrdU Antibody, clone 131-14871
clone 131-14871, Chemicon®, from mouse
Synonim(y):
BrdU
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
131-14871, monoclonal
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Chemicon®
metody
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
izotyp
IgG1
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
Powiązane kategorie
Opis ogólny
The thymidine analog 5-bromo-2′-deoxyuridine (BrdU) is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. BrdU is readily incorporated into newly synthesized DNA, labeling cells that have progressed through the S-phase of the cell cycle and thereby serving as a marker for proliferating cells.
Specyficzność
Recognizes BrdU (Bromodeoxyuridine).
Zastosowanie
Anti-BrdU Antibody, clone 131-14871 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, IHC, IHC(P). This bromodeoxyuridine antibody is expect to react in all species.
Immunohistochemistry on deparaffinized sections of kidney, duodenum, and liver tissue: 1:1000-1:1250.
Flow cytometry on 100μL of 106 cells: 1:5000.
Optimal dilutions must be determined by the end user.
IMMUNOHISTOCHEMICAL PROCEDURE
FOR PARAFFIN EMBEDDED TISSUE SECTIONS
STAINING PROCEDURE:
1. Deparaffinize sections in xylene 3 x 5 minutes. Resin embedded or GMA (Glycol methacrylate, 2-hydroxyethyl methacrylate) treated sections it is unnecessary to deparaffinize as there is no paraffin.
2. Place slides in graded alcohols (100, 95, 90%) to water at 2 minute intervals, and air dry. (Omit for GMA sections)
3. Circle sections with PAP pen or diamond pen to identify and dry thoroughly.
4. For GMA or resin sections, place slides in 0.5% Tween/PBS (pH 7.6) for 29 minutes.
5. Incubate in prewarmed (40°C) 1N HCl for 1 hour.
6. Wash 3 x 3 minutes with PBS (pH 7.6). Slides may be held at this point and the procedure continued the following day.
7. Incubate in 1X Trypsin solution (0.02-0.05% w/v, prewarmed to 40°C) for 20 minutes at room temperature for NBF (normal buffered formalin) fixed tissue or 10 minutes for Carnoy′s fixed tissue.
8. Wash 3 x 5 minutes with PBS (pH 7.6).
9. Incubate sections in 1% H2O2 (10 mL 30% H2O2 in 290 mL methanol) for 20 minutes, or GMA sections in 3% H2O2 (10 mL 30% H2O2 in 90 mL water) for 3 minutes.
10. Wash 2 x 2 minutes with PBS (pH 7.6).
11. Using the Vector ABC kit, add 3 drops of normal horse serum to 10 mL of PBS. Use this solution to block the slides for 20 minutes at room temperature.
12. Blot the slides of excess normal horse serum and incubate with MAB4072 diluted in PBS/BSA/Tween 20 for 1 hour at room temperature.
13. Wash 3 x 3 minutes with PBS (pH 7.6).
14. Add 3 drops of normal horse serum to 10 mL of PBS and then add 1 drop of biotinylated antibody stock. Incubate the slides with this diluted second antibody for 30 minutes at room temperature.
15. Prepare the Vectastain ABC Reagent by adding 2 drops of reagent A to 5 mL of PBS. Then add 2 drops of Reagent B to the same mixing bottle. Allow to sit for about 30 minutes prior to use.
16. Wash 3 x 3 minutes with PBS (pH 7.6).
17. Incubate the slides with ABC Reagent for 30 minutes at room temperature.
18. Wash 3 x 3 minutes with PBS (pH 7.6).
19. Prepare the substrate by mixing an equal volume of 0.02% H2O2 (made in distilled water from a 30% stock solution) and 0.1% DAB made in 0.1 M Tris buffer, pH 7.2. The H2O2 should be freshly prepared from concentrated stock. Because many peroxide substrates are unstable in the presence of H2O2 or when exposed to light, the substrate should be prepared just prior to use. Since DAB is a suspected carcinogen, care should be taken in handling and disposing of all peroxidase substrates.
20. Stain the slides with DAB substrate for 2-5 minutes.
21. Wash 2 x 2 minutes with distilled water.
22. Counterstain with hemotoxylin, dehydrate through xylene, and mount the coverslip.
Flow cytometry on 100μL of 106 cells: 1:5000.
Optimal dilutions must be determined by the end user.
IMMUNOHISTOCHEMICAL PROCEDURE
FOR PARAFFIN EMBEDDED TISSUE SECTIONS
STAINING PROCEDURE:
1. Deparaffinize sections in xylene 3 x 5 minutes. Resin embedded or GMA (Glycol methacrylate, 2-hydroxyethyl methacrylate) treated sections it is unnecessary to deparaffinize as there is no paraffin.
2. Place slides in graded alcohols (100, 95, 90%) to water at 2 minute intervals, and air dry. (Omit for GMA sections)
3. Circle sections with PAP pen or diamond pen to identify and dry thoroughly.
4. For GMA or resin sections, place slides in 0.5% Tween/PBS (pH 7.6) for 29 minutes.
5. Incubate in prewarmed (40°C) 1N HCl for 1 hour.
6. Wash 3 x 3 minutes with PBS (pH 7.6). Slides may be held at this point and the procedure continued the following day.
7. Incubate in 1X Trypsin solution (0.02-0.05% w/v, prewarmed to 40°C) for 20 minutes at room temperature for NBF (normal buffered formalin) fixed tissue or 10 minutes for Carnoy′s fixed tissue.
8. Wash 3 x 5 minutes with PBS (pH 7.6).
9. Incubate sections in 1% H2O2 (10 mL 30% H2O2 in 290 mL methanol) for 20 minutes, or GMA sections in 3% H2O2 (10 mL 30% H2O2 in 90 mL water) for 3 minutes.
10. Wash 2 x 2 minutes with PBS (pH 7.6).
11. Using the Vector ABC kit, add 3 drops of normal horse serum to 10 mL of PBS. Use this solution to block the slides for 20 minutes at room temperature.
12. Blot the slides of excess normal horse serum and incubate with MAB4072 diluted in PBS/BSA/Tween 20 for 1 hour at room temperature.
13. Wash 3 x 3 minutes with PBS (pH 7.6).
14. Add 3 drops of normal horse serum to 10 mL of PBS and then add 1 drop of biotinylated antibody stock. Incubate the slides with this diluted second antibody for 30 minutes at room temperature.
15. Prepare the Vectastain ABC Reagent by adding 2 drops of reagent A to 5 mL of PBS. Then add 2 drops of Reagent B to the same mixing bottle. Allow to sit for about 30 minutes prior to use.
16. Wash 3 x 3 minutes with PBS (pH 7.6).
17. Incubate the slides with ABC Reagent for 30 minutes at room temperature.
18. Wash 3 x 3 minutes with PBS (pH 7.6).
19. Prepare the substrate by mixing an equal volume of 0.02% H2O2 (made in distilled water from a 30% stock solution) and 0.1% DAB made in 0.1 M Tris buffer, pH 7.2. The H2O2 should be freshly prepared from concentrated stock. Because many peroxide substrates are unstable in the presence of H2O2 or when exposed to light, the substrate should be prepared just prior to use. Since DAB is a suspected carcinogen, care should be taken in handling and disposing of all peroxidase substrates.
20. Stain the slides with DAB substrate for 2-5 minutes.
21. Wash 2 x 2 minutes with distilled water.
22. Counterstain with hemotoxylin, dehydrate through xylene, and mount the coverslip.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Postać fizyczna
Format: Purified
Liquid in 10 mM Phosphate buffer, pH 7.4 containing 150 mM NaCl and 0.1% sodium azide.
Protein A purified
Przechowywanie i stabilność
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Komentarz do analizy
Control
Brdu treated cells
Brdu treated cells
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Visualization of nascent transcripts on Drosophila polytene chromosomes using BrUTP incorporation.
W Y Chang et al.
BioTechniques, 29(5), 934-936 (2000-11-21)
Peng-Wei Tseng et al.
Frontiers in genetics, 13, 816955-816955 (2022-04-12)
Unlike gonochoristic fishes, sex is fixed after gonadal differentiation (primary sex determination), and sex can be altered in adults (secondary sex determination) of hermaphroditic fish species. The secondary sex determination of hermaphroditic fish has focused on the differences between testicular
Helge Rask-Andersen et al.
Hearing research, 203(1-2), 180-191 (2005-04-28)
Time lapse video recordings of cultured adult human and guinea pig spiral ganglion (hSG and gpSG) show that mitogen responsive progenitor/stem cells develop in the form of spheres that proliferate and differentiate into mature neurons and glia cells. Neurospheres, cultured
Ahmad Galuta et al.
Frontiers in neuroscience, 14, 607-607 (2020-07-07)
Improving the clinical translation of animal-based neural stem/progenitor cell (NSPC) therapies to humans requires an understanding of intrinsic human and animal cell characteristics. We report a novel in vitro method to assess spinal cord NSPCs from a small (rodent) and
Artem K Velichko et al.
Nucleic acids research, 43(13), 6309-6320 (2015-06-03)
Heat stress is one of the best-studied cellular stress factors; however, little is known about its delayed effects. Here, we demonstrate that heat stress induces p21-dependent cellular senescence-like cell cycle arrest. Notably, only early S-phase cells undergo such an arrest
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej