10280879001

5-Bromo-2′-deoxyuridine

>97%, crystalline solid, pkg of 1 g

• Synonim(y): 5-Bromo-2′-deoxyuridine, brdu, 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
• Wzór empiryczny (zapis Hilla): C₉H₁₁BrN₂O₅
• Numer CAS: 59-14-3
• Masa cząsteczkowa: 307.10
• Beilstein: 30395
• Numer MDL: MFCD00006529
• Kod UNSPSC: 12352204
• Identyfikator substancji w PubChem: 329749004

Właściwości

• Poziom jakości: 100
• Próba: >97%
• Postać: crystalline solid
• masa cząsteczkowa: 307.1
• opakowanie: pkg of 1 g
• producent / nazwa handlowa: Roche
• mp: 191-194 °C (dec.) (lit.)
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• ciąg SMILES: OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• Klucz InChI: WOVKYSAHUYNSMH-RRKCRQDMSA-N

Opis

Opis ogólny

It is conventionally used as a marker for S-phase synthesis of DNA.

Zastosowanie

5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.

Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.

Działania biochem./fizjol.

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

Jakość

Purity: 97% (from N), chromatographically homogeneous

Uwaga dotycząca przygotowania

Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.

Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H340,H361fd
• Zwroty wskazujące środki ostrożności: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Klasyfikacja zagrożeń: Muta. 1B - Repr. 2
• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable