Przejdź do zawartości
Merck
Wszystkie zdjęcia(2)

Key Documents

MAB2059

Sigma-Aldrich

Anti-Integrin β4 Antibody, clone ASC-8

clone ASC-8, Chemicon®, from mouse

Synonim(y):

CD104, MAB2059Z

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych

Wybierz wielkość


Wybierz wielkość

Zmień widok

About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

ASC-8, monoclonal

reaktywność gatunkowa

human

producent / nazwa handlowa

Chemicon®

metody

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

izotyp

IgG1κ

Powiązane kategorie

Specyficzność

Human beta4 integrin (CD104)

Immunogen

Human squamous cell carcinoma cells (SCC9)

Zastosowanie

Immunoprecipitation: A representative lot of this antibody clone precipitated PAGE bands of 66, 76, 120, 135 and 200 kDa (reduced) or 66, 110, 125, 130 and 190 kDa (non-reduced) from surface-biotinylated SCC9 cells.

Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue).

Flow Cytometry: A representative lot of this antibody clone stained human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

Functional Activity Assay: A representative lot of this antibody clone induced a sub-basal split at the basement membrane in non-wounded tissue. In addition, wound closure was significantly inhibited by ASC-8, as the epithelial tongue only covered 40 percent of the wound area at 120 hours post-wounding. These results demonstrate that the β4 adhesion-blocking antibody may have adverse effects on normal tissue (Egles, C., et al., 2010).

Working dilutions must be determined by end user.
Anti-Integrin β4 Antibody, clone ASC-8 is an antibody against Integrin β4 for use in FC, IP, IH.
Research Category
Cell Structure
Research Sub Category
Integrins

Jakość

Flow Cytometry Analysis:
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 A431 cells.
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 HeLa cells.

Powiązanie

Replaces: 05-283

Postać fizyczna

Format: Purified
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Przechowywanie i stabilność

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.

Nie możesz znaleźć właściwego produktu?  

Wypróbuj nasz Narzędzie selektora produktów.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Combinatorial fibronectin and laminin signaling promote highly efficient cardiac differentiation of human embryonic stem cells.
Sa, S; Wong, L; McCloskey, KE
BioResearch Open Access null
Iris Eke et al.
Molecular cancer research : MCR, 16(12), 1855-1864 (2018-07-26)
Adaptation of tumor cells to radiotherapy induces changes that are actionable by molecular targeted agents and immunotherapy. This report demonstrates that radiation-induced changes in integrin expression can be targeted 2 months later. Integrins are transmembrane cell adhesion molecules that are
Mirko Nowak et al.
Biomaterials, 112, 20-30 (2016-10-16)
Matrix systems used to study complex three-dimensional (3D) cellular processes like mammary epithelial tissue morphogenesis and tumorigenesis ex vivo often require ill-defined biological components, which lead to poor reproducibility and a lack of control over physical parameters. In this study, a
Daisuke Nanba et al.
The Journal of cell biology, 209(2), 305-315 (2015-04-22)
Image-based identification of cultured stem cells and noninvasive evaluation of their proliferative capacity advance cell therapy and stem cell research. Here we demonstrate that human keratinocyte stem cells can be identified in situ by analyzing cell motion during their cultivation.
Integrins as antimetastatic targets of RGD-independent snake venom components in liver metastasis [corrected].
Rosenow, F; Ossig, R; Thormeyer, D; Gasmann, P; Schluter, K; Brunner, G; Haier, J; Eble, JA
Neoplasia null

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej