ABS314
Anti-APPL1 Antibody
from rabbit, purified by affinity chromatography
Synonim(y):
DCC-interacting protein 13-alpha, Dip13-alpha, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
rat, mouse, human
metody
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... APPL1(26060)
Opis ogólny
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1 (APPL1) is also called DCC-interacting protein 13-alpha (Dip13-alpha). APPL1 is necessary for cell proliferation in response to extracellular signals and links Rab5 to nuclear signal transduction. APPL1 is expressed at high levels in heart, ovary, pancreas, and skeletal muscle tissues.
Immunogen
Recombinant protein corresponding to human APPL1.
Zastosowanie
Detect Adapter protein containing PH domain, PTB domain & leucine zipper motif 1 using this rabbit polyclonal antibody, Anti-APPL1 Antibody validated for use in western blotting, IP, ICC & IHC.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of L6 and NIH/3T3 cell lysate.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated APPL1 in HUVEC cell lysate (Lin, D. C., et al. (2006). Mol Cell Biol. 26(23):8928-8941.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in liver tissues from mice fed with either a standard diet, high fat diet, or a high fat diet with exercise (Marinho, R., et al. (2012). J Cell Physiol. 227(7):2917-2926.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in pancreatic islets and impaired GSIS cells of dietary and obese mice (Cheng, K. K., et al. (2009). Cell Metab. 9(5):417-427.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated APPL1 in HUVEC cell lysate (Lin, D. C., et al. (2006). Mol Cell Biol. 26(23):8928-8941.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in liver tissues from mice fed with either a standard diet, high fat diet, or a high fat diet with exercise (Marinho, R., et al. (2012). J Cell Physiol. 227(7):2917-2926.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in pancreatic islets and impaired GSIS cells of dietary and obese mice (Cheng, K. K., et al. (2009). Cell Metab. 9(5):417-427.).
Jakość
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of A431 cell lysate.
Opis wartości docelowych
~84 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Postać fizyczna
Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
APPL1 associates with TrkA and GIPC1 and is required for nerve growth factor-mediated signal transduction.
Lin, Dan C, et al.
Molecular and cellular biology, 26, 8928-8941 (2006)
Kenneth K Y Cheng et al.
Cell metabolism, 9(5), 417-427 (2009-05-07)
Hepatic insulin resistance is the major contributor to fasting hyperglycemia in type 2 diabetes. Here we report that the endosomal adaptor protein APPL1 increases hepatic insulin sensitivity by potentiating insulin-mediated suppression of the gluconeogenic program. Insulin-stimulated activation of Akt and
R Marinho et al.
Journal of cellular physiology, 227(7), 2917-2926 (2011-09-23)
Hepatic insulin resistance is the major contributor to fasting hyperglycemia in type 2 diabetes. The protein kinase Akt plays a central role in the suppression of gluconeogenesis involving forkhead box O1 (Foxo1) and peroxisome proliferator-activated receptor gamma co-activator 1 alpha
Aparajita Lahree et al.
Cell reports, 39(9), 110886-110886 (2022-06-02)
Intracellular pathogens manipulate host cells to survive and thrive. Cellular sensing and signaling pathways are among the key host machineries deregulated to favor infection. In this study, we show that liver-stage Plasmodium parasites compete with the host to sequester a
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