553506-M

RAP, Human, Recombinant, E. coli

• Synonim(y): Receptor Associated Protein
• Kod UNSPSC: 12352202

Właściwości

• Próba: ≥90% (SDS-PAGE)
• Poziom jakości: 100
• Postać: liquid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; avoid repeated freeze/thaw cycles
• temp. przechowywania: −70°C

Opis

Opis ogólny

Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.

Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

150 mM NaCl, 100 mM Tris-HCl, pH 7.4.

Rekonstytucja

Following initial thaw, aliquot and freeze (-70°C).

Inne uwagi

Li, Y., et al. 2002. Biochemistry41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160.
Anderson, O.M., et al. 2001. Biochemistry40, 15408.
Bu, G. 2001. Int. Rev. Cytol.209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable