14-338
ROKα/ROCK-II Protein, active, rat, 10 µg
Active, rat, N-terminal, His-tagged, fusion protein corresponding to amino acids 2-543 of rat ROKa/ROCK-II. For use in Kinase Assays.
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About This Item
Kod UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.32
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
masa cząsteczkowa
Mw 66 kDa
producent / nazwa handlowa
Upstate®
metody
activity assay: suitable (kinase)
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
informacje o genach
rat ... ROCK1(81762)
Opis ogólny
N-terminal, His-tagged, fusion protein corresponding to amino acids 2-543 of rat ROKa/ROCK-II.
Note: The preparation contains numerous Sf21 cellular proteins which bind weakly to Ni-NTA agarose.
Note: The preparation contains numerous Sf21 cellular proteins which bind weakly to Ni-NTA agarose.
Product Source: Rat ROKa/ROCK-II expressed in Sf21 insect cells.
Działania biochem./fizjol.
Protein Target: ROKα/ROCK-II
Target Sub-Family: AGC
Jakość
Rroutinely evaluated using the S6 Kinase Assay Kit
Postać fizyczna
10μg of enzyme in 2.3μl of 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 2mM benzamidine, 0.4mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni-NTA agarose
Przechowywanie i stabilność
6 months at -70°C
Inne uwagi
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Skin Sens. 1
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
J Feng et al.
The Journal of biological chemistry, 274(6), 3744-3752 (1999-01-28)
Rho-associated kinase (Rho-kinase) from chicken gizzard smooth muscle was purified to apparent homogeneity (160 kDa on SDS-polyacrylamide gel electrophoresis) and identified as the ROKalpha isoform. Several substrates were phosphorylated. Rates with myosin phosphatase target subunit 1 (MYPT1), myosin, and the
David A Carlson et al.
Cell chemical biology, 25(10), 1195-1207 (2018-07-24)
Sustained vascular smooth muscle hypercontractility promotes hypertension and cardiovascular disease. The etiology of hypercontractility is not completely understood. New therapeutic targets remain vitally important for drug discovery. Here we report that Pim kinases, in combination with DAPK3, regulate contractility and
M Eto et al.
The Journal of biological chemistry, 276(31), 29072-29078 (2001-06-09)
Histamine stimulus triggers inhibition of myosin phosphatase-enhanced phosphorylation of myosin and contraction of vascular smooth muscle. In response to histamine stimulation of intact femoral artery, a smooth muscle-specific protein called CPI-17 (for protein kinase C-potentiated inhibitory protein for heterotrimeric myosin
Vincent Sauzeau et al.
Methods in molecular biology (Clifton, N.J.), 1527, 213-218 (2017-01-25)
Increased arterial tone and the resulting rise in peripheral vascular resistance are major determinants of the elevated arterial pressure in hypertension. The RhoA/Rho kinase signaling pathways are now recognized as a major regulator of vascular smooth muscle contraction and arterial
E Sahai et al.
Current biology : CB, 9(3), 136-145 (1999-02-18)
The Ras-related GTPase RhoA controls signalling processes required for cytoskeletal reorganisation, transcriptional regulation, and transformation. The ability of RhoA mutants to transform cells correlates not with transcription but with their ability to bind ROCK-I, an effector kinase involved in cytoskeletal
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