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05-1572

Sigma-Aldrich

Anti-MDC1 Antibody, clone P2B11

clone P2B11, from mouse

Synonim(y):

Nuclear factor with BRCT domains 1, homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint 1

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

P2B11, monoclonal

reaktywność gatunkowa

mouse

reaktywność gatunkowa (przewidywana na podstawie homologii)

chimpanzee (based on 100% sequence homology), bovine (based on 100% sequence homology), human (based on 100% sequence homology)

metody

immunocytochemistry: suitable
western blot: suitable

izotyp

IgG1κ

numer dostępu NCBI

NP_001010833.2

numer dostępu UniProt

Q5PSV9

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... MDC1(9656)
mouse ... Mdc1(240087)

Opis ogólny

MDC1 is a subunit of the cohesin complex and is an essential protein required for sister chromatid cohesion in mitosis and meiosis. Temperature-sensitive mutations of this protein have been associated with defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation. Its expression is cell cycle regulated and peaks in S phase. Expression of MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 has been shown to interact with H2A.X near sites of DNA double-strand breaks and recruit ATM kinase to the damaged region of DNA. Consistent with this role, MDC1 also appears to modulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.

Specyficzność

This antibody recognizes MDC1 at and around the N-terminus.

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

Zastosowanie

Anti-MDC1 Antibody, clone P2B11 is a Mouse Monoclonal Antibody for detection of MDC1 also known as Nuclear factor with BRCT domains 1 & has been validated in ICC & WB.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MDC1 in NIH/3T3 cells.

Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Lou, Z., et al. (2003). Nature. 421(6926):957-961.)

Opis wartości docelowych

~ 184 kDa There are 4 isoforms of human MDC1 because of splicing with molecular weights of approximately 227, 198, 196, and 117 kDa

Postać fizyczna

Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) without 0.05% sodium azide.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Alexandra Mayer et al.
Cell cycle (Georgetown, Tex.), 15(4), 546-558 (2016-01-09)
Because low levels of DNA double strand breaks (DSBs) appear not to activate the ATM-mediated prophase I checkpoint in full-grown oocytes, there may exist mechanisms to protect chromosome integrity during meiotic maturation. Using live imaging we demonstrate that low levels
Hung-Sheng Shang et al.
Oncology reports, 36(4), 2207-2215 (2016-08-09)
Cervical cancer is one of the most common cancers in women worldwide and it is a prominent cause of cancer mortality. Curcumin is one of the major compounds from Turmeric and has been shown to induce cytotoxic cell death in
Yung-Luen Shih et al.
Oncology reports, 36(4), 2094-2100 (2016-08-31)
Casticin, a polymethoxyflavone, has been demonstrated to possess anticancer activities, yet no study has shown in detail that casticin induces DNA damage in lung cancer cells. The purpose of this study was to investigate the possible molecular mechanisms of casticin
Kuntian Luo et al.
Genes & development, 30(23), 2581-2595 (2016-12-13)
Homologous recombination (HR) is one of the major DNA double-strand break (DSB) repair pathways in mammalian cells. Defects in HR trigger genomic instability and result in cancer predisposition. The defining step of HR is homologous strand exchange directed by the
Yunhui Li et al.
Nature communications, 8, 15752-15752 (2017-06-02)
BRCA1 regulates multiple cellular pathways that maintain genomic stability including cell cycle checkpoints, DNA repair, protein ubiquitination, chromatin remodelling, transcriptional regulation and apoptosis. Receptor-associated protein 80 (RAP80) helps recruit BRCA1 to double-strand breaks (DSBs) through the scaffold protein CCDC98 (Abraxas)
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