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MABN68

Sigma-Aldrich

Anti-PSD95 Antibody, clone K28/43

clone K28/43, from mouse

Synonim(y):

Postsynaptic density protein 95, Synapse-associated protein 90, Tax interaction protein 15, discs large homolog 4, discs, large homolog 4 (Drosophila), post-synaptic density protein 95

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

K28/43, monoclonal

reaktywność gatunkowa

rat

reaktywność gatunkowa (przewidywana na podstawie homologii)

mouse (based on 100% sequence homology), human (immunogen homology)

metody

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

izotyp

IgG2aκ

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... DLG4(1742)

Opis ogólny

PSD95 (postsynaptic density 95) represent a class of anchoring proteins which serve to localize various neuronal ion channels to post-synaptic densities. PSD95 and related proteins contain PDZ domains, which interact with NMDA receptors and voltage-gated K+ channels via a ET/SXV motif in the cytoplasmic carboxyl terminus of the receptor channels.

Immunogen

Recombinant protein corresponding to human PSD95.

Zastosowanie

Detect PSD95 using this Anti-PSD95 Antibody, clone K28/43 validated for use in IH, WB & IC.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected PSD95 in rat cortex tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Maeda, T., et al. (2005). Invest. Ophthalmol. Vis. Sci. 46(11):4320-4327.)
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Jakość

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PSD95 on 10 µg of rat brain tissue lysate.

Opis wartości docelowych

~95 kDa observed.

Powiązanie

Replaces: 04-1066

Postać fizyczna

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Rat brain tissue lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Lu Yang et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 24(48), 10846-10857 (2004-12-03)
The specification and organization of glutamatergic synaptic transmission require the coordinated interaction among glutamate receptors and their synaptic adaptor proteins closely assembled in the postsynaptic density (PSD). Here we investigated the interaction between NMDA receptors and metabotropic glutamate receptor 5
Vibeke M Bruinenberg et al.
Nutrients, 8(4), 185-185 (2016-04-23)
The inherited metabolic disease phenylketonuria (PKU) is characterized by increased concentrations of phenylalanine in the blood and brain, and as a consequence neurotransmitter metabolism, white matter, and synapse functioning are affected. A specific nutrient combination (SNC) has been shown to
Optimized protocol for retinal wholemount preparation for imaging and immunohistochemistry.
Ivanova, E; Toychiev, AH; Yee, CW; Sagdullaev, BT
Journal of Visualized Experiments null
Min-Jian Wang et al.
Medical science monitor : international medical journal of experimental and clinical research, 25, 4627-4638 (2019-07-04)
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Pranesh Padmanabhan et al.
eLife, 8 (2019-06-27)
The Src kinase Fyn plays critical roles in memory formation and Alzheimer's disease. Its targeting to neuronal dendrites is regulated by Tau via an unknown mechanism. As nanoclustering is essential for efficient signaling, we used single-molecule tracking to characterize the

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