AB10022
Anti-Histone H2A.X Antibody
from rabbit, purified by affinity chromatography
Synonim(y):
Histone H2A.X, Histone H2AX, H2a/x, Histone H2A.X, H2AFX
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
mouse, human, rat, bovine
producent / nazwa handlowa
Chemicon®
Upstate®
metody
flow cytometry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
bovine ... H2Ax(531733)
human ... H2AX(3014)
mouse ... H2Ax(15270)
rat ... H2Ax(500987)
Powiązane kategorie
Opis ogólny
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2A.X is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition, this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins at a damaged site.
Immunogen
Epitope: a.a. 121-139
KLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.X.
Zastosowanie
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-Histone H2A.X Antibody, Human (Rabbit Polyclonal Antibody) validated in WB to detect Histone H2A.X also known as H2AFX.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected 500 ng of recombinant Histone H2A.X.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of Rat2 acid extract, BALB 3T3/A31 acid extract, and calf thymus histone extract tissue lysate.
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Histone H2A.X in 1X10E6 HeLa cells.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of Rat2 acid extract, BALB 3T3/A31 acid extract, and calf thymus histone extract tissue lysate.
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Histone H2A.X in 1X10E6 HeLa cells.
Jakość
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of HeLa acid extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of HeLa acid extract.
Opis wartości docelowych
~16 kDa observed
Postać fizyczna
Peptide affinity chromatography
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Telomeres are favoured targets of a persistent DNA damage response in ageing and stress-induced senescence.
Hewitt, G; Jurk, D; Marques, FD; Correia-Melo, C; Hardy, T; Gackowska, A; Anderson et al.
Nature Communications null
Kristine Lillebø Holm et al.
PloS one, 12(10), e0185708-e0185708 (2017-10-04)
In the present study, we address the important issue of whether B-cells protected from irradiation-induced cell death, may survive with elevated levels of DNA damage. If so, such cells would be at higher risk of gaining mutations and undergoing malignant
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