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199303
Resazurin sodium salt
certified by the BSC, certified by the Biological Stain Commission
Synonim(y):
7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
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About This Item
Wzór empiryczny (zapis Hilla):
C12H6NNaO4
Numer CAS:
Masa cząsteczkowa:
251.17
Beilstein:
3794971
Numer WE:
Numer MDL:
Kod UNSPSC:
12171500
Identyfikator substancji w PubChem:
NACRES:
NA.47
Polecane produkty
klasa czystości
certified by the Biological Stain Commission
agency
certified by the BSC
Formularz
powder
skład
Dye content, 75%
rozpuszczalność
1 M NH4OH: 1 mg/mL
λmaks.
380 nm (2nd)
598 nm
Zastosowanie
diagnostic assay manufacturing
hematology
histology
temp. przechowywania
room temp
ciąg SMILES
[Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1
InChI
1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
Klucz InChI
IVGPGQSSDLDOLH-UHFFFAOYSA-M
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Opis ogólny
Resazurin is a small N-oxide derivative of dihydroxyphenoxazone. It is a non-ionic dye containing a small and planar chromophore. It is a redox indicator and changes from bluish violet through pink to colorless upon reduction. It is also used as a pH indicator for Ph 3.5 to 6.5 wherein it changed from orange to bluish violet. It is commercially available as resazurin sodium salt, which is also known as 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide, sodium salt. It forms the red, fluorescent hydroxyphenoxazone resorufin on reduction and colorless hydroresorufin on further reduction.
Zastosowanie
For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
Resazurin is mostly used as a biological redox indicator. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). Its applications include:
- the measurement of metabolic activity and the proliferation of living cells
- the detection of bacterial reduction in milk
- in viability assays as a component of culture media for the detection of viable bacteria
- to determine the number of motile sperm in sheep semen
- to assay lactate dehydrogenase
- as an analytical reagent to quantify L-glutamate
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Organy docelowe
Respiratory system
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
dust mask type N95 (US), Eyeshields, Gloves
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