Przejdź do zawartości
Merck
Wszystkie zdjęcia(3)

Key Documents

View All Documentation

11836170001

Roche

cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail

Protease Inhibitor Cocktail Tablets provided in a glass vial, Tablets provided in a glass vial

Synonim(y):

protease inhibitor, protease inhibitor cocktail, roche protease inhibitor

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(3)

About This Item

Kod UNSPSC:
12352204

Postać

tablet

zastosowanie

sufficient for 25 10mL extraction solution preparations

opakowanie

vial of 25 tablets

producent / nazwa handlowa

Roche

metody

inhibition assay: suitable

rozpuszczalność

aqueous buffer: soluble

temp. przechowywania

2-8°C

Powiązane kategorie

Opis ogólny

cOmplete, Mini, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets, they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).
Due to the optimized composition of the tablets, they show excellent inhibition of serine and cysteine proteases, and well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.

cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.

Specyficzność

For the inhibition of serine, cysteine, but not metalloproteases.

Zastosowanie

cOmplete™, Mini, EDTA-free protease inhibitor cocktail has been used for the inhibition of serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.
cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.
cOmplete™Mini, EDTA-free Protease Inhibitor Cocktail has used as a component of
  • resuspension buffer for nuclear lysates preparation from prostate cancer cell lines for chromatin and RNA immunoprecipitation
  • resuspension buffer for protein extraction from neurospheres for western blot analysis
  • homogenization buffer for protein extraction from rat left ventricle samples and immunoprecipitaion buffer for human embryonic kidney 293 (HEK293) cells

Cechy i korzyści

Use cOmplete, Mini Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.

  • Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
  • Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
  • Complete protection: Instantly protect your proteins against a broad range of proteases.
  • Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.

Komponenty

Proprietary mixture of several protease inhibitors.

Jakość

Performance-tested with pancreas extract.

Postać fizyczna

Tablets in glass vials

Uwaga dotycząca przygotowania

Working concentration: 1 tablet per 10 ml extraction solution
Working solution: Preparation of Working Solution:

One cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.

Stock solution (7 X conc.):

Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Rekonstytucja

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C, or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informacje prawne

cOmplete is a trademark of Roche
This page may contain text that has been machine translated.

najczęściej kupowane z tym produktem

Numer produktu
Opis
Cennik

Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H315,H319

Klasyfikacja zagrożeń

Eye Irrit. 2 - Skin Irrit. 2

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Risk SNP-mediated promoter-enhancer switching drives prostate cancer through lncRNA PCAT19
Hua JT, et al.
Cell, 174(3), 564-575 (2018)
Patrick R Punch et al.
Glycobiology, 30(7), 446-453 (2020-01-04)
High-dose irradiation poses extreme risk of mortality from acute damage to the hematopoietic compartment and gastrointestinal tract. While bone marrow transplantation can reestablish the hematopoietic compartment, a more imminent risk of death is posed by gastrointestinal acute radiation syndrome (GI-ARS)
Da Eun Kwak et al.
PloS one, 15(1), e0227618-e0227618 (2020-01-11)
Alzheimer's disease (AD) is an ageing-related neurodegenerative disease characterized and diagnosed by deposition of insoluble amyloid-β (Aβ) plaques in the brain. The plaque accumulation in the brain directly affects reduced levels of Aβ in cerebrospinal fluid (CSF) and blood, as
Junwei Zeng et al.
Nature communications, 11(1), 3990-3990 (2020-08-12)
The molecular mechanisms regulating lymphocyte homing into lymph nodes are only partly understood. Here, we report that B cell-specific deletion of the X-linked gene, Cosmc, and the consequent decrease of protein O-glycosylation, induces developmental blocks of mouse B cells. After
Donald B Jump
Methods in molecular biology (Clifton, N.J.), 579, 375-389 (2009-09-19)
Very long chain fatty acids confer functional diversity on cells by variations in their chain length and degree of unsaturation. Microsomal fatty acid elongation represents the major pathway for determining the chain length of saturated, monounsaturated, and polyunsaturated fatty acids
Wyświetl wszystkie powiązane dokumenty

Produkty

Roche Protein Stabilization Reagents

Get maximum protection after protein isolation by using protease and phosphatase inhibitor cocktail tablets from Roche.

Protokoły

Protokół cOmplete™, Mini, bez EDTA

Rozpuścić jedną tabletkę w 10 ml buforu wodnego lub wody. W przypadku wysokiej aktywności proteolitycznej rozpuścić jedną tabletkę w 7 ml buforu.

Powiązane treści

Liza i ekstrakcja białek

Metody lizy komórek i ekstrakcji białek obejmują przegląd różnych technik, od rozpuszczania detergentami po mechaniczne rozbijanie, wspierając potrzeby badawcze.

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Liza i ekstrakcja białek

Metody lizy komórek i ekstrakcji białek obejmują przegląd różnych technik, od rozpuszczania detergentami po mechaniczne rozbijanie, wspierając potrzeby badawcze.

Wybór inhibitora proteazy: Unikanie utraty białek podczas przygotowywania próbek

Wybierz różne typy inhibitorów proteaz w zależności od potrzeb, aby zapobiec degradacji białek podczas izolacji i charakteryzacji oraz zabezpieczyć białka podczas przygotowywania próbek.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej