RIP
Imprint® RNA Immunoprecipitation Kit
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
동의어(들):
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
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모든 사진(5)
About This Item
UNSPSC 코드:
41116158
NACRES:
NA.54
추천 제품
Quality Level
일반 설명
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.
Learn more about this product and view application data.
Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
애플리케이션
Imprint® RNA Immunoprecipitation Kit has been used for:
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
특징 및 장점
- Xtra Protein A magnetic beads with higher binding capacity
- Step-by-step protocols for cell lysis and immunoprecipitation
- RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
- Negative control and antibody included
법적 정보
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
키트 구성품 역시 별도로 이용 가능함
제품 번호
설명
SDS
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderSDS
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderSDS
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionSDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS
- R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μLSDS
필요하나 제공되지 않음
제품 번호
설명
가격
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point (°F)
100.4 °F
Flash Point (°C)
38 °C
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Huijuan Xiao et al.
Cell & bioscience, 8, 23-23 (2018-03-29)
The acquisition of drug resistance has been considered as a main obstacle for cancer chemotherapy. Tumor protein 53 target gene 1 (TP53TG1), a p53-induced lncRNA, plays a vital role in the progression of human cancers. However, little is known about
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Oncotarget, 8(64), 107859-107869 (2018-01-04)
Long non-coding RNA PTENP1, the pseudogene of PTEN tumor suppressor, was previously reported to be a tumour suppressor in some cancer types. However, the precise effects mediated by PTENP1 transcripts within intricate regulatory networks involving molecular interactions with PTEN and
Nicola Amodio et al.
Leukemia (2018-03-01)
The biological role and therapeutic potential of long non-coding RNAs (lncRNAs) in multiple myeloma (MM) are still to be investigated. Here, we studied the functional significance and the druggability of the oncogenic lncRNA MALAT1 in MM. Targeting MALAT1 by novel
Lin Cao et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 93, 570-579 (2017-07-08)
Non-small cell lung cancer (NSCLC), known as the most common type of lung cancer, has caused great economic losses and brought the patients with NSCLC great suffering. The NSCLC chemo-resistance to cisplatin (DDP) -based chemotherapy remains a huge challenge. The
문서
Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit
프로토콜
Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
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