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Merck
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Key Documents

P8340

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use with mammalian cell and tissue extracts, DMSO solution

동의어(들):

Protease Inhibitor Mix

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About This Item

MDL number:
UNSPSC 코드:
12352200
NACRES:
NA.77

product name

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

생물학적 소스

bovine lung

Quality Level

형태

DMSO solution

저장 온도

−20°C

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일반 설명

Protease Inhibitor Cocktail for mammalian cell and tissue extracts, in DMSO solution. It contains six individual components for inhibiting serine, cysteine, and acid proteases, and aminopeptidases.

특이성

Inhibits serine, cysteine, and acid proteases, and aminopeptidases.

애플리케이션

Ideal for use as a buffer component during sonication, lysis buffer, and RIPA buffer

Provides protection against endogenous enzyme activity in cell and tissue extracts

생화학적/생리학적 작용

This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases.

특징 및 장점

Broad-spectrum inhibition: inhibits multiple classes of proteases and aminopeptidases

Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C

Consistent performance: ready-to-use solution

성분

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

주의사항

This cocktail should be stored at -20 °C.

제조 메모

One mL of this cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g of bovine liver or 10 mL of lysate from CHO cells. This product is supplied as a solution in DMSO.

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

188.6 °F - closed cup

Flash Point (°C)

87 °C - closed cup


시험 성적서(COA)

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