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Merck
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Key Documents

P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

동의어(들):

Protease inhibitor solution

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About This Item

MDL number:
UNSPSC 코드:
12352200
NACRES:
NA.77

product name

Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

생물학적 소스

synthetic

Quality Level

형태

DMSO solution

저장 온도

−20°C

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일반 설명

DMSO solution designed for use in the purification of histidine-tagged proteins.

The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

특이성

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

애플리케이션

The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.

It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.

특징 및 장점

Specifically formulated for histidine-tagged protein purification.

Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.

Compatibility with IMAC applications due to omission of chelating agents.

Supplied in convenient packaging options.

성분

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

수량

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

제조 메모

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

185.0 °F - closed cup

Flash Point (°C)

85 °C - closed cup


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문서 라이브러리 방문

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E Boudreau et al.
The EMBO journal, 19(13), 3366-3376 (2000-07-06)
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