D7295

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

• 동의어(들): Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs
• UNSPSC 코드: 41106305
• NACRES: NA.52

속성

• Quality Level: 300
• 형태: liquid
• 농도: 10 mM
• 색상: colorless
• 응용 분야: agriculture
• 외래 활성: DNase, RNase, none detected
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

애플리케이션

dNTP Mix has been used:

• in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
• in reverse transcription of total RNA to cDNA.
• as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
• routine and long PCR
• manual and automated DNA sequencing
• cDNA synthesis and labeling reactions

특징 및 장점

• Purity of each dNTP: Minimum 99%
• Conveniently formulated; 1 μL is used per 50 μL PCR
• Equimolar amounts of each dNTP means less pipetting
• Minimize risk of contamination in PCR
• UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable