TAQ-RO

Taq DNA Polymerase, 5 U/μl

optimum pH ~9.0 (20 °C), optimum reaction temp. 72 °C

• 동의어(들): dna amplification, pcr, polymerase, pcr, primer extension, dna amplification, primer extension
• 효소 위원회 번호: 2.7.7.7 (BRENDA, IUBMB)
• UNSPSC 코드: 41106300

속성

• 생물학적 소스: bacterial (Thermus aquaticus)
• Quality Level: 100
• 재조합: expressed in E. coli
• Grade: Molecular Biology
• 형태: liquid
• 사용: sufficient for ≤1,000 reactions (11146173001); sufficient for ≤10,000 reactions (11435094001); sufficient for ≤2,000 reactions (11418432001); sufficient for ≤5,000 reactions (11596594001); sufficient for ≤200 reactions (11146165001)
• 특이 활성도: 5 U/μL
• 분자량: 95 kDa
• 포장: pkg of 1,000 U (11418432001 [4 x 250 U]); pkg of 2,500 U (11596594001 [10 x 250 U]); pkg of 5,000 U (11435094001 [20 x 250 U]); pkg of 100 U (11146165001); pkg of 500 U (11146173001 [1 x 500 U])
• 제조업체/상표: Roche
• 파라미터: 72 °C optimum reaction temp.
• 기술: DNA sequencing: suitable; PCR: suitable
• 색상: colorless
• 최적 pH: ~9.0 (20 °C)
• solubility: water: miscible
• 적합성: suitable for molecular biology
• NCBI 수납 번호: AAA27507
• UniProt 수납 번호: P19821
• 응용 분야: genomic analysis; life science and biopharma
• 외래 활성: Nicking activity 30 units, none detected; Ribonuclease 30 units, none detected; Unspecific endonucleases 30 units, none detected
• 저장 온도: −20°C

설명

일반 설명

Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme was cloned in E.coli.

애플리케이션

Taq DNA Polymerase can be used in simple, routine PCR. Roche Applied Science Taq DNA polymerase is held to rigorous purity and quality standards. This preparation of recombinant Taq DNA Polymerase can be applied for:

• PCR
• RT-PCR
• qPCR
• Other primer-extension reactions, such as sequencing and labeling

특징 및 장점

Reliable reproducible results:
High lot-to-lot consistency.
No need to test each lot:
Taq DNA Polymerase is rigorously tested.
Prevent PCR carryover:
dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.

포장

1 kit containing 2 components

품질

Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.

단위 정의

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 60 min at +65 °C under the assay conditions given above.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl₂, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α^-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 5 U/μl

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

키트 구성품 전용

• Taq DNA Polymerase 5 U/μl
• PCR Buffer with MgCl₂ 10x concentrated
• MgCl₂ Stock Solution
• PCR Buffer without MgCl₂

안전성 정보

• 유해 및 위험 성명서: H412
• 예방조치 성명서: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash