M3148

2-Mercaptoethanol

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

• 동의어(들): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein: 773648
• EC Number: 200-464-6
• MDL number: MFCD00004890
• UNSPSC 코드: 12161504
• PubChem Substance ID: 24896800
• NACRES: NA.31

속성

• Grade: for molecular biology
• Quality Level: 200
• vapor density: 2.69 (vs air)
• vapor pressure: 1 mmHg ( 20 °C)
• 제품 라인: BioReagent
• 분석: 99% (GC/titration)
• 형태: liquid
• expl. lim.: 18 %
• 반응 적합성: reagent type: reductant
• 농도: 14.3 M (pure liquid)
• 기술: cell culture | mammalian: suitable; electrophoresis: suitable
• refractive index: n20/D 1.500 (lit.)
• pH: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• solubility: H₂O: soluble 1 mL/mL
• density: 1.114 g/mL at 25 °C (lit.)
• 외래 활성: DNase, RNase, protease, none detected
• 저장 온도: room temp
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

설명

애플리케이션

Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:

• as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
• to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
• to trypsinize mouse embryonic stem cells

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

안전성 정보

• 픽토그램: GHS05,GHS06,GHS08,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410
• 예방조치 성명서: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): 154.4 °F - closed cup
• Flash Point (°C): 68 °C - closed cup
• 개인 보호 장비: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter