G9284

Guanidine hydrochloride solution

Colorless liquid, 7.8 - 8.3 M, pH- 4.5 - 5.5

• 동의어(들): Aminoformamidine hydrochloride, Guanidinium chloride, Guanidium chloride
• 실험식(Hill 표기법): CH₅N₃ · HCl
• CAS Number: 50-01-1
• Molecular Weight: 95.53
• Beilstein: 3591990
• MDL number: MFCD00013026
• UNSPSC 코드: 12352107
• PubChem Substance ID: 24895369
• NACRES: NA.55

속성

• 형태: liquid
• density: 1.18 g/mL at 25 °C (lit.)
• SMILES string: Cl.NC(N)=N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• 유전자 정보: human ... KCNA1(3736) , KCNA10(3744) , KCNA2(3737) , KCNA3(3738) , KCNA4(3739) , KCNA5(3741) , KCNA6(3742) , KCNA7(3743) , KCNB1(3745) , KCNB2(9312) , KCNC1(3746) , KCNC2(3747) , KCNC3(3748) , KCNC4(3749) , KCND1(3750) , KCND2(3751) , KCND3(3752) , KCNF1(3754) , KCNG1(3755) , KCNG2(26251) , KCNG3(170850) , KCNG4(93107) , KCNH1(3756) , KCNH2(3757) , KCNH3(23416) , KCNH4(23415) , KCNH5(27133) , KCNH6(81033) , KCNH7(90134) , KCNH8(131096) , KCNQ1(3784) , KCNQ2(3785) , KCNQ3(3786) , KCNQ4(9132) , KCNQ5(56479) , KCNS1(3787) , KCNS2(3788) , KCNS3(3790) , KCNV1(27012) , KCNV2(169522)

설명

애플리케이션

Guanidine hydrochloride solution has been used:

• for protein denaturation
• to homogenize hypothalamus, thalamus, prefrontal cortex, and brainstem samples for total β-amyloid (Aβ) extraction and Aβ42 quantification
• as a chaotropic denaturant to treat human immunodeficiency virus-1 (HIV-1) samples to study its effects on HIV-1 sensitivity and to assay protein stability

생화학적/생리학적 작용

Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step. GnHCl can be used to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon. Gnd-HCl is not used in proteomics experiments due to its interference with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS). It is appropriate for performing rapid and single-step affinity-purification mass spectrometry (AP-MS) experiments.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302 + H332,H315,H319
• 예방조치 성명서: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter