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Merck
모든 사진(4)

Key Documents

50950

Sigma-Aldrich

Guanidine hydrochloride

≥98%

동의어(들):

Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

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About This Item

Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
Molecular Weight:
95.53
Beilstein:
3591990
EC Number:
MDL number:
UNSPSC 코드:
12352107
PubChem Substance ID:
NACRES:
NA.55

Quality Level

분석

≥98%

형태

powder or crystals

저장 조건

(Tightly closed. Dry. )

색상

colorless to white

pH

(25 °C, 4.6 - 6/573 g/L)

mp

180-185 °C (lit.)

solubility

H2O: 6 M, clear (100 Hazen)

density

1.3 g/cm3 (lit.)

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Guanidine hydrochloride, or aminoformamidine hydrochloride, is a versatile chemical compound widely used in cell biology and biochemical research due to its potent chaotropic properties. Its ability to disrupt protein and nucleic acid structures makes it valuable for purifying proteins and nucleic acids, particularly mRNA. Guanidine hydrochloride unfolds proteins, facilitating their purification and refolding, and it is effective in solubilizing denatured insoluble proteins at higher concentrations.

At lower concentrations, guanidine hydrochloride has the intriguing ability to promote the refolding of denatured proteins, aiding in protein renaturation studies. In RNA extraction, it acts as a strong denaturant, disrupting cell structures and ensuring the integrity of extracted RNA by inactivating RNA enzymes. Overall, guanidine hydrochloride′s denaturing and renaturing properties make it an essential reagent for various cell biology applications, including protein purification, nucleic acid isolation, and protein refolding studies.

애플리케이션

Guanidine hydrochloride has been used:
  • for lysing homogenized brain tissue
  • in the preparation of incubation buffer for Ni-sepharose protein binding, purification, propionylation, and on-bead digestion to minimize nonspecific binding to the affinity resin
  • in the preparation of solutions/extraction buffer to extract soluble protein from the human tissue

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

생화학적/생리학적 작용

Guanidine hydrochloride, an efficient chaotropic reagent, is involved in RNA isolation, globular protein denaturation, and protein refolding studies. This denaturant is appropriate for performing rapid and single-step affinity-purification mass spectrometry (AP-MS) experiments.

특징 및 장점

Highly versatile surfactant for your cell biology and biochemical research

기타 정보

For additional information on our range of Biochemicals, please complete this form.

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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