50983

Guanidine thiocyanate solution

BioUltra, for molecular biology, ~6 M in H₂O

• 동의어(들): Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate
• Linear Formula: NH₂C(=NH)NH₂ · HSCN
• CAS Number: 593-84-0
• Molecular Weight: 118.16
• Beilstein: 3563461
• MDL number: MFCD00013027
• UNSPSC 코드: 12352107
• PubChem Substance ID: 57651292
• NACRES: NA.25

속성

• Grade: for molecular biology
• 제품 라인: BioUltra
• 농도: ~6 M in H₂O
• 불순물: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.474-1.483
• pH: 4.5-7.0 (25 °C)
• 음이온 미량물: chloride (Cl^-): ≤500 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• 양이온 미량물: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤500 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• 흡수: cut-off at 280 nm
• SMILES string: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• InChI key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

설명

일반 설명

Guanidine thiocyanate is a chaotropic agent that functions as a strong protein denaturant. It is widely used in RNA isolation protocols. Guanidine thiocyanate can irreversibly inactivate RNase and DNase and breaks cellular membranes. It dissolves proteins by disturbing their secondary structures. Guanidine thiocyanate also separates nucleoproteins from nucleic acids and aids the digestion of the proteins with proteases. It improves the activity of proteinase K thus making it the protease of choice.

애플리케이션

Guanidine thiocyanate solution has been used to extract:

• plasma RNA from human immunodeficiency virus (HIV)-infected and control patients
• total RNA from collected tissues (shoots, flowers, fruits, and seeds)
• in reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA from teratocarcinoma cell line (Tera-1)

주의사항

possibly crystallized product can be dissolved again by slightly heating to 40-50 °C.

안전성 정보

• 픽토그램: GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H302 + H312 + H332,H314,H412
• 예방조치 성명서: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• 보충제 위험성: EUH032
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter