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Merck
모든 사진(1)

주요 문서

X100

Sigma-Aldrich

Triton X-100

laboratory grade

동의어(들):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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About This Item

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
UNSPSC 코드:
12161900
PubChem Substance ID:
NACRES:
NA.21

생물학적 소스

synthetic

Quality Level

Grade

laboratory grade

설명

non-ionic

양식

liquid

분자량

micellar avg mol wt 80,000
average mol wt 625

응집 번호

100-155

CMC

0.2-0.9 mM (20-25°C)

mp

6  °C (43 °F)

전이 온도

cloud point 65 °C
pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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일반 설명

Triton X-100 has hemolytic property and is used for DNA extraction.
Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

애플리케이션

Triton-X has been used:
  • In the permeabilization of cells for immunofluorescence staining
  • As a component of lysis buffer in western blot analysis
  • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

생화학적/생리학적 작용

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

특징 및 장점

  • Ideal for Cell Biology and Biochemical research applications
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

기타 정보

For additional information on our range of Biochemicals, please complete this form.

법적 정보

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

픽토그램

CorrosionExclamation markEnvironment

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

483.8 °F - closed cup

Flash Point (°C)

251 °C - closed cup

개인 보호 장비

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)


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시험 성적서(COA)

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문서 라이브러리 방문

이미 열람한 고객

Growth and hydration of Triton X-100 micelles in monovalent alkali salts: a light scattering study
Molina-Bolivar JA, et al.
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Blood-feeding ectoparasites constitute a growing burden for human and animal health, and animal production worldwide. In particular, mites (Acari: Gamasida) of the genera Dermanyssus (Dermanyssidae) and Ornithonyssus (Macronyssidae) infest birds and cause gamasoidosis in humans. The tropical fowl mite, Ornithonyssus
Bryan D Hudson et al.
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Inhibitors of Wnt signaling have been shown to be involved in prostate cancer (PC) metastasis; however the role of Sclerostin (Sost) has not yet been explored. Here we show that elevated Wnt signaling derived from Sost deficient osteoblasts promotes PC

프로토콜

Enzymatic Assay of Cholesterol Oxidase

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

This page shows how to solubilize membrane proteins with products from GE Healthcare.

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