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Merck
모든 사진(4)

주요 문서

X100RS

Sigma-Aldrich

Triton X-100 reduced

동의어(들):

Polyoxyethylene (10) isooctylcyclohexyl ether

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About This Item

Linear Formula:
4-(C8H17)C6H10(OCH2CH2)nOH, n~10
CAS Number:
MDL number:
UNSPSC 코드:
12161900
NACRES:
NA.25

생물학적 소스

synthetic (oragnic)

Quality Level

설명

non-ionic

양식

viscous liquid

분자량

micellar average mol wt 80,000
average mol wt 625

응집 번호

100-155

refractive index

n20/D 1.473 (lit.)

CMC

0.2-0.9

mp

48 -50  °C (118 - 122 °F)

전이 온도

cloud point 65 °C

solubility

water: soluble, clear to slightly hazy, colorless

density

1.029 g/mL at 25 °C (lit.)

흡수

≤0.25 at 277 nm in H2O at 0.5%

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)C1CCC(CC1)OCCOCCOCCOCCOCCOCCOCCO

InChI

1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3

InChI key

QQJNBKDKLMCALZ-UHFFFAOYSA-N

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일반 설명

Triton X-100 reduced is a versatile nonionic surfactant featuring a hydrophilic polyethylene oxide chain, commonly employed as a laboratory detergent. Its applications span various purposes, including cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a vital component in lysis buffers. Widely utilized in biochemical and cell biology research, Triton X-100 facilitates the isolation of lipid rafts, enhancing the solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, this product exhibits excellent wetting properties and emulsification enhancement.

In cell biology, Triton X-100 plays a crucial role in solubilizing membrane-bound proteins, preserving their native conformation in solution. Furthermore, Triton X-100 reduced, resulting from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative, has been associated with potential benefits in enzyme digestion enhancement and effects on the photoisomerization of bacteriorhodopsin. The versatility of Triton X-100 in various research applications underscores its significance as a fundamental tool in biochemical and cell biology investigations.

애플리케이션

Triton X-100 reduced has been used:
  • in nuclear magnetic resonance (NMR) sample tube for lysing/rupturing the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles
  • in cell permeabilization
  • as a component in Tris/HCl to determine hydrolysis of the esters for enzyme activity assays

특징 및 장점

  • Non-ionic surfactant
  • Improves solubility and dispersibility of substances
  • Excellent wetting properties
  • Enhances emulsification
  • High purity product for research applications

제조 메모

Hydrogenated to reduce UV absorbance.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

법적 정보

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

호환 제품

제품 번호
설명
가격

픽토그램

Exclamation markEnvironment

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

235.4 °F - closed cup

Flash Point (°C)

113 °C - closed cup

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


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이미 열람한 고객

Slide 1 of 2

1 of 2

H A Van Lith et al.
European journal of biochemistry, 206(2), 527-535 (1992-06-01)
1. Substrate hydrolysis by two purified rabbit liver esterase-1 allozymes (ES-1A and ES-1B) was compared under conditions differing in substrate, pH and temperature. ES-1A and ES-1B activities had a similar pH and temperature dependency and similar thermal stability profile. 2.
Luke H Chamberlain
FEBS letters, 559(1-3), 1-5 (2004-02-28)
The relative insolubility of lipid rafts in cold non-ionic detergents is the most widely used method to purify these fascinating membrane domains from intact cells or membranes. Most of what we know about lipid raft function has been derived from
Mammalian long-chain acyl-CoA synthetases.
Soupene E, Kuypers FA.
Exp. Biol. Med, 233, 507-521 (2008)
Mehul B Shah et al.
Methods in molecular biology (Clifton, N.J.), 398, 21-28 (2008-01-25)
Raft and caveolar microdomains have been proposed to participate in numerous cellular functions including signal transduction, cholesterol trafficking, and vesicular sorting. Traditional methods of isolation of rafts from cultured cells and tissue samples have exploited the biochemical properties of these
Naomi Takahashi et al.
The Journal of comparative neurology, 525(1), 204-230 (2016-08-31)
Global inhibition is a fundamental physiological mechanism that has been proposed to shape odor representation in higher-order olfactory centers. A pair of mushroom bodies (MBs) in insect brains, an analog of the mammalian olfactory cortex, are implicated in multisensory integration

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