T8787

Triton^™ X-100

for molecular biology

• 동의어(들): t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• CAS Number: 9036-19-5
• Beilstein: 2315025
• MDL number: MFCD00128254
• UNSPSC 코드: 12161900
• PubChem Substance ID: 329826885
• NACRES: NA.21

속성

• 생물학적 소스: synthetic (organic)
• Quality Level: 200
• Grade: for molecular biology
• 설명: non-ionic
• 형태: liquid
• 분자량: micellar avg mol wt 80,000; average mol wt 625
• 응집 번호: 100-155
• 기술: protein purification: suitable; protein quantification: suitable; western blot: suitable
• 불순물: <1.00% water (Karl Fischer)
• pH: 9.7
• CMC: 0.2-0.9 mM (20-25°C)
• mp: 6 °C
• 전이 온도: cloud point 65 °C; pour point ~7 °C
• solubility: water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
• density: 1.06 g/mL at 25 °C (lit.)
• 양이온 미량물: Fe: <5 ppm; K: <0.05%; Na: <0.1%; heavy metals: <5 ppm
• HLB: 13.5
• 응용 분야: hematology; histology
• 외래 활성: DNase and RNase, none detected
• 저장 온도: room temp
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

설명

일반 설명

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

애플리케이션

Triton^™ X-100 has been used:

• In immunohistochemistry for staining the Flat-mount retinas
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
• To permeabilise cells during Immunofluorescent microscopic studies
• As a positive control in LDH assay to determine the cell membrane integrity
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
• For the preparation of outer membrane protein exctract
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells
• In the treatment of tissue sections for Immunofluorescence labeling

생화학적/생리학적 작용

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

특징 및 장점

• Ideal for sensitive molecular biology research applications
• Tested for DNase and RNase
• Non-ionic nondenaturing detergent
• Superior wetting agent and emulsifier
• Highly versatile surfactant

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

For additional information on our range of Biochemicals, please complete this form.

법적 정보

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

안전성 정보

• 픽토그램: GHS05,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H318,H410
• 예방조치 성명서: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): 483.8 °F - closed cup
• Flash Point (°C): 251 °C - closed cup
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter