43816

DL-Dithiothreitol solution

BioUltra, for molecular biology, ~1 M in H₂O

• 동의어(들): Cleland′s reagent, (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
• 실험식(Hill 표기법): C₄H₁₀O₂S₂
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• Beilstein: 1719757
• MDL number: MFCD00004877
• UNSPSC 코드: 12352201
• PubChem Substance ID: 57650196
• NACRES: NA.25

속성

• Grade: for molecular biology
• Quality Level: 200
• 제품 라인: BioUltra
• 분석: 900-1100 mmol/L (Titration molarity)
• 형태: liquid
• 반응 적합성: reagent type: reductant
• 농도: ~1 M in H₂O
• 불순물: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 2.7-5.0
• density: 1.04 g/mL at 20 °C
• 흡수: cut-off at 330 nm
• 적합성: in accordance for filter test; suitable for molecular biology
• 저장 온도: 2-8°C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

설명

일반 설명

Dithiothreitol (DTT), a thiol group protectant, is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.

애플리케이션

DL-dithiothreitol solution has been used:

• in the treatment of intestinal explants
• in homogenate preparation of gill cells for enzymatic assays
• in protein electrophoresis for western blotting
• in transglutaminase activity
• in sample buffer for SDS-PAGE
• in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing
• as a component of protein extraction buffer for cell lysis

생화학적/생리학적 작용

DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.Dithiothreitol (DTT) is broadly utilized in synthetic peptide synthesis and biochemical arrangements of thiol proteins. It can also be used to study protein chemistry topics like enzyme activity and protein folding. DTT explicitly controls the thiol-disulfide exchange response to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. Thiols and the cyclic disulfide of DTT are the products of this reaction.

특징 및 장점

• BioUltra grade - 1M DTT solution, suitable for molecular biology
• Free from DNases, RNases, phosphatases, and proteases

기타 정보

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안전성 정보

• 픽토그램: GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H315,H318
• 예방조치 성명서: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
• Hazard Classifications: Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter