추천 제품
Quality Level
분석
≥98% (HPLC)
≥99.0% (titration)
형태
powder
반응 적합성
reagent type: reductant
색상
white
mp
41-44 °C (lit.)
solubility
H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow
응용 분야
general analytical
저장 온도
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
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일반 설명
Dithiothreitol (DTT) is a disulfide (SH) reducing agent found in proteins and in the amino acid cysteine. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange. It is almost 7-fold stronger reducing agent than βME, hence efficient in lower concentrations.
애플리케이션
DTT has been used:
- as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk.
- as a component of medium for the demembranation and reactivation of spermatozoa.
- to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.
- as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.
- in proteomics analysis as in-solution protein digestion for mass spectrometry
- as a buffer component for protein quantification, to prepare wash buffer, lysis buffer, sample buffer, and protein elution buffer
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
생화학적/생리학적 작용
Dithiothreitol (DTT) is extensively applied in chemical peptide synthesis and biochemical preparations of thiol proteins. It is also used in protein chemistry studies, such as protein folding and enzyme activity. It also acts as an enzyme stabilizer to stop DNA modification. DTT specifically mediates the thiol-disulfide interchange reaction to entirely reduce the intra- or inter-molecular disulfide bonds in biomolecules. This reaction results in the formation of thiols and the cyclic disulfide of DTT.
특징 및 장점
- High-quality DTT (HPLC≥98%), (titration≥99.0%)
- Suitable for electrophoresis, proteomics analysis
기타 정보
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호환 제품
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
이미 열람한 고객
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Auditory neuroscience, 1, 101-109 (1994-01-01)
Vestibular dark cell epithelium was isolated from the semicircular canal of gerbils to test the proposal that the sulfhydryl alkylating agent N-ethylmaleimide (NEM) inhibits K(+) secretion by this tissue and does so by reacting with a site in or near
Scandinavian journal of immunology, 60(5), 486-493 (2004-11-16)
Alphas1-Casein (CAS1_BOVIN), the major allergen of cow's milk (CM), is widely used as hydrolysates in infant diet formulae and additive to other processed food items. To date, most of the reported B-cell epitope mapping were performed on polyethylene pins or
Reproduction (Cambridge, England), 124(5), 713-719 (2002-11-06)
This study investigated the effects of different environmental conditions on the motility parameters of paddlefish (Polyodon spathula) spermatozoa. Paddlefish spermatozoa demonstrated the following characteristics: (i) all spermatozoa were motile 10 s after activation with a velocity of 130-160 microm s(-1);
Clinical chemistry, 39(3), 500-503 (1993-03-01)
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