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HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl with Acetonitrile Mobile Phase

HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl with Acetonitrile Mobile Phase application for HPLC

材料

分析カラム

製品番号
詳細
価格

53348-U

Ascentis® Express フェニルヘキシル、2.7 μm HPLCカラム

2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

標準

製品番号
詳細
価格

D7282

デスメチルジアゼパム

analytical standard

O-902

オキサゼパム 溶液

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

A-903

アルプラゾラム 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-907

クロナゼパム 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

D-907

ジアゼパム 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U)

mobile phase

[A] water; [B] acetonitrile; (62:38, A:B)

flow rate

1.4 mL/min

pressure

2600 psi (179 bar)

column temp.

35 °C

detector

UV, 250 nm

injection

2 μL

sample

0.1 g/mL of each in 20% methanol

詳細

アナリシスノート

The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS