X0629

Anti-XRCC1 ウサギ宿主抗体

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-X-ray repair complementing defective repair in Chinese hamster cells 1
• MDL番号: MFCD01865396
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 85 kDa
• 化学種の反応性: human
• 濃度: ~1 mg/mL
• テクニック: indirect immunofluorescence: 5-10 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton; western blot: 0.5-1 μg/mL using A431 cell lysates
• UniProtアクセッション番号: P18887
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... XRCC1(7515) ; mouse ... Xrcc1(22594) ; rat ... Xrcc1(84495)

詳細

詳細

X-ray repair cross-complementing group 1 (XRCC1) is a testis enriched protein. It is considered as a DNA repair gene.

免疫原

synthetic peptide corresponding to amino acids 615-633 of human XRCC1, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is identical in mouse and rat.

アプリケーション

Anti-XRCC1 antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• indirect immunofluorescence and telomere FISH (TEL-FISH)
• protein detection
• western analysis

生物化学的／生理学的作用

X-ray repair cross-complementing group 1 (XRCC1) is a molecular scaffold protein that coordinates the assembly of repair complexes at damaged sites. XRCC has been shown to physically interact with several enzymes known to be involved in the repair of SSBs, including DNA ligase IIIa, DNA polymerase b, APE1, polynucleotide kinase (PNK), poly (ADP-ribose), polymerases 1 and 2 (PARP-1 and 2) and others. It participates in the DNA base excision repair (BER) pathway.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)