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由来生物
rabbit
結合体
unconjugated
抗体製品の状態
IgG fraction of antiserum
抗体製品タイプ
primary antibodies
クローン
polyclonal
形状
buffered aqueous solution
テクニック
immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins
western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
詳細
Anti-VP16 is produced in rabbit using a synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue. Whole antiserum is fractionated and further purified by ionexchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
免疫原
synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue.
アプリケーション
Anti-VP16 recognizes VP16 fusion proteins by immunoblotting and immunoprecipitation. It is used to study the effect of sialic acid on herpes simplex virus type 1 envelope glycoproteins. It is also used to study if self-association of lymphocytic choriomeningitis virus nucleoprotein is mediated by its N-terminal region and is not required for its anti-interferon function.
物理的形状
0.01 MPBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
V4388-VAR:
IXO11270:
V4388-BULK:
V4388-200UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Lei Guo et al.
PloS one, 7(9), e45749-e45749 (2012-10-03)
ICP22 is a multifunctional herpes simplex virus 1 (HSV-1) immediate early protein that functions as a general repressor of a subset of cellular and viral promoters in transient expression systems. Although the exact mechanism of repression remains unclear, this protein
Jamie Snider et al.
Nature protocols, 5(7), 1281-1293 (2010-07-03)
The biological function of proteins may be predicted by identification of their interacting partners, and one of the major goals of the postgenomic era is the mapping of protein interaction networks. Membrane proteins are of particular interest because of their
Health care's future.
R J Nowak
Ohio medicine : journal of the Ohio State Medical Association, 87(5), 229-230 (1991-05-01)
Ida G Lunde et al.
The Journal of physiology, 582(Pt 3), 1277-1287 (2007-04-28)
The effects of exercise on skeletal muscle are mediated by a coupling between muscle electrical activity and gene expression. Several activity correlates, such as intracellular Ca(2+), hypoxia and metabolites like free fatty acids (FFAs), might initiate signalling pathways regulating fibre-type-specific
Kate Sokolina et al.
Molecular systems biology, 13(3), 918-918 (2017-03-17)
G-protein-coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex biochemical features. To obtain a global
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