コンテンツへスキップ
Merck
すべての画像(1)

主要文書

安全性情報

G3042

Sigma-Aldrich

Anti-GAL4 DNA-BD antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

別名:

Anti-Galactose 4 DNA Binding Domain

ログイン組織・契約価格を表示する


About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.56

由来生物

rabbit

品質水準

結合体

unconjugated

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

フォーム

buffered aqueous solution

テクニック

western blot: 1-2 μg/mL using GAL4 (DBD 1-147) fusion protein

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

詳細

The polyclonal Anti-GAL4 DNA-BD rabbit antibody is a synthetic peptide that recognizes GAL4 DNA binding domain fusion proteins. GAL4 protein is an 881 amino acid transcription factor. It contains functionally independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a constituent of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).

特異性

Anti-GAL4 DNA-BD recognizes GAL4 DNA binding domain fusion proteins.

免疫原

Saccharomyces cerevisiaeのGAL4タンパク質DNA結合ドメイン(アミノ酸39~52)に相当する合成ペプチド。

アプリケーション

Anti-GAL4 DNA-BD antibody produced in rabbit has been used in western blotting.

生物化学的/生理学的作用

GAL4 transcription factor is involved in the induction of genes that regulate galactose metabolism in Saccharomyces cerevisiae. The yeast two hybrid system is widely used for the characterization of domains that are necessary and sufficient for the interaction of two known proteins by deletion and mutational analysis.

物理的形状

0.01 M PBS溶液(pH 7.4, 1%BSA, 15 mMアジ化ナトリウム含有)。

保管および安定性

For continuous use store at 2-8°C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください

保管分類コード

12 - Non Combustible Liquids

WGK

nwg

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

G3042-BULK:
G3042-200UG:
G3042-VAR:


最新バージョンのいずれかを選択してください:

試験成績書(COA)

Lot/Batch Number

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Sirtuin 7-dependent deacetylation of DDB1 regulates the expression of nuclear receptor TR4
Karim Mdf, et al.
Biochemical and biophysical research communications, 490(2), 423-428 (2017)
Lidia Hurtado et al.
Plant molecular biology, 62(1-2), 291-304 (2006-07-18)
Arabidopsis thaliana BRAHMA (BRM, also called AtBRM) is a SNF2 family protein homolog of Brahma, the ATPase of the Drosophila SWI/SNF complex involved in chromatin remodeling during transcription. Here we show that, in contrast to its Drosophila counterpart, BRM is
Yukiko Imai et al.
Chromosoma, 126(6), 681-695 (2017-05-21)
PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. This role involves its DNA-binding domain, which is composed of a tandem array of zinc fingers, and PRDM9-dependent
Mitsuki Uemura et al.
Veterinary and comparative oncology, 18(2), 247-255 (2019-09-14)
RAD51 forms a complex with BRCA2 and plays a central role in the DNA damage response pathway that is associated with homologous recombination. The structures of RAD51 and its homologues are highly conserved from prokaryotes to higher eukaryotes. Although a
X Wang et al.
Oncogene, 27(13), 1894-1904 (2007-10-02)
As a cyclin-dependent kinase inhibitor, p16(INK4a) plays a key role in cell cycle progression and cellular differentiation, and its expression is frequently altered in human cancers through epigenetically mediated transcriptional silencing. In this report, we demonstrate that p300 was able

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)