ABE476

Anti-GAL4 Antibody (activation domain)

from rabbit, purified by affinity chromatography

• 別名: Regulatory protein GAL4
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: yeast
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_015076
• UniProtアクセッション番号: P04386
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: Saccharomyces cerevisiae ... Gal4(855828)

詳細

詳細

GAL4 is a transcriptional activator which controls the expression of genes involved in the catabolism of galactose. In addition to a DNA-binding domain, Gal4 contains two acidic regions: activating region I (aa148-238) and activating region II (aa768-881), which can both activate transcription when independently joined to the DNA-binding domain. The activation domains of Gal4 have been shown to interact with Gal80, TBP, SUG1, and ADA2, among other general transcription factors.

特異性

This antibody recognizes the C-terminus of GAL4 (activation domain).

免疫原

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of yeast GAL4 (activation domain).

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
細胞周期、DNA複製及び修復

Use Anti-GAL4 (activation domain) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect GAL4 (activation domain) also known as Regulatory protein GAL4.

品質

Evaluated by Western Blot in Gal4 positive and Gal4 negative yeast cell lysate.

Western Blot Analysis: A 1:160,000 dilution of this antibody detected Gal4 in 5 mg of Gal4 positive (+ recombinant Gal4) yeast cell lysate. This antibody also detected small mounts of endogenous Gal4 in Gal4 negative (- recombinant Gal4) yeast cell lysate.

ターゲットの説明

~28 kDa, ~38 kDa, ~77 kDa observed

関連事項

Replaces: 06-283

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Gal4 positive and Gal4 negative yeast

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable