T6824

pPolh-FLAG-1 トランスファーベクター

• 別名: ddddk tag, dykddddk tag
• UNSPSCコード: 41106621
• NACRES: NA.32

特性

• 品質水準: 200
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

The pPolh-FLAG-1 vector contains the strong viral polyhedrin (polh) promoter for high-level expression of target genes during the very late phase of infection. The vector also contains a high copy bacterial origin of replication and an ampicillin resistance gene (ampr) for easy propagation in Escherichia coli host cells. Following co-transfection with linear baculovirus DNA into insect cells, allelic replacement between homologous viral sequences (AcNPV ORF 603 and ORF 1629) in the vector and the baculovirus DNA transfers the FLAG-target gene fusion sequence into the viral genome.

アプリケーション

昆虫細胞での組み替えバキュロウイルスの作製や、N末端FLAG^®融合タンパク質の発現に用いるトランスファーベクターです。

法的情報

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)