STR1

Enhanced Avian 一本鎖合成キット

Components for cDNA synthesis with enhanced AMV reverse transcriptase

• 別名: Reverse Transcriptase cDNA synthesis kit, eAMV-RT
• UNSPSCコード: 12352200
• NACRES: NA.55

特性

• 品質水準: 200
• 使用法: sufficient for 50 reactions; kit sufficient for 50 reactions
• 特徴: dNTPs included; hotstart: no
• テクニック: RT-PCR: suitable
• 色: colorless
• 入力: purified RNA
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)⁺ RNA.

Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.

アプリケーション

Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).

単位の定義

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)_12-18 as primer.

法的情報

本製品の購入は研究のみを目的とし、サ-マルサイクラ-を使ってポリメラ-ゼ連鎖反応(PCR)処理を実施する場合に限ってその使用が認められます。PCRの自動化処理におけるサ-マルサイクラ-の利用は、Applied Biosystems社への支払いまたはライセンスを受けたサ-マルサイクラ-の購入による、ロイヤルティ-の前払いによってカバ-されます。

キットの構成要素のみ

• Enhanced Avian Reverse Transcriptase 1000 U

キットの構成要素は別途購入可能です。

• Deoxynucleotide mix 50 μL
• O4387 Anchored oligo (dT)₂₃ 100 μL
• R7647 Random nonamers 100 μL
• O4387 Ribonuclease inhibitor 50 μL
• W1754 PCR grade water 1.5 mL

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable